Genome Wide Computational Identification of Tuna (Thunnus orientalis) MicroRNAs and Their Targets
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Applying genome-wide computational-based approaches (using the draft genome sequence published in recent years) and following a set of strict filtering criteria, a total of 48 potentially conserved microRNAs belonging to 30 families were identified from economically important fish tuna (Thunnus orientalis). Using BLAST and RNA hybrid program a total of 19 potential miRNA targets were also identified in this study, which includes a number of signaling molecules (serine/threonine-protein kinase, GTPase activating protein etc.) and transcription factors (F-box protein, zinc finger protein etc.). This study provides the basis for gaining a better understanding of miRNA-mediated gene regulatory processes in fishes.
Keywordsteleost fish Pacific bluefin tuna (Thunnus orientalis) comparative genomics MFE MFEI
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- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acid S 41:95–98Google Scholar
- Nakamura Y, Mori K, Saitoh K, Oshima K, Mekuchi M, Sugaya T, Shigenobu Y, Ojima N, Muta S, Fujiwara A, Yasuike M, Oohara I, Hirakawa H, Chowdhury VS, Kobayashi T, Nakajima K, Sano M, Wada T, Tashiro K, Ikeo K, Hattori M, Kuhara S, Gojobori T, Inouye K (2013) Evolutionary changes of multiple visual pigment genes in the complete genome of Pacific bluefin tuna. Proc Natl Acad Sci USA 110:11061–11066. doi:10.1073/pnas.1302051110CrossRefGoogle Scholar