Adaptation responses of individuals to environmental changes in the ciliate Euplotes crassus
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Although the response unit of living organisms to environmental changes is at the individual level, most experiments on the adaptation responses of ciliates have been conducted in batches, comprising multiple-individuals, due to their microscopic size. However, here, we confirmed that individuals undergo different division cycles in monocultures of Euplotes crassus. They also exhibited transcript variations of 4.63-fold in SSU and of 22.78- fold in Hsp70. Additionally, in salt-stressed E. crassus individuals, SSU transcripts of individuals varied by 6.92-fold at 27 psu, 8.69- fold at 32 psu, and 2.51-fold at 37 psu. However, the maximum difference in Hsp70 was only 4.23-fold under all conditions. These results suggest there may be different biological rhythms even in siblings derived from the same parent. It can also be inferred that various environmental factors have different effects on different E. crassus individuals. Therefore, to elucidate relationships between organism adaptations and environmental changes, studies at the individual level should be conducted with multi-individual approaches.
Key wordsadaptation ciliates environmental change gene expression individual difference
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- Fenchel T (1987) Ecology of protozoa: the biology of free-living phagotrophic protists. Springer-Verlag, Heidelberg, 197 pGoogle Scholar
- Hausmann K, Bradbury PC (1996) Ciliates: cells as organisms. Gustav Fischer Verlag, Heidelberg, 485 pGoogle Scholar
- Lynn DH (2008) The ciliated protozoa: characterization, classification, and guide to the literature. Springer Science & Business Media, Heidelberg, 605 pGoogle Scholar
- Newell RC (2013) Adaptation to environment: essays on the physiology of marine animals. Elsevier, Pennsylvania, 554 pGoogle Scholar
- Orr PT, Rasmussen JP, Burford MA, Eaglesham GK, Lennox SM (2010) Evaluation of quantitative real-time PCR to characterise spatial and temporal variations in cyanobacteria, Cylindrospermopsis raciborskii (Woloszynska) Seenaya et Subba Raju and cylindrospermopsin concentrations in three subtropical Australian reservoirs. Harmful Algae 9:243–254CrossRefGoogle Scholar
- Raikov IB (1969) The macronucleus of ciliates. In: Chen TT (ed) Research in protozoology. Pergamon Press, London, pp 1–128Google Scholar
- Seyfert HM, Hipke H, Schmidt W (1984) Isolation and phenotypic characterization of Tetrahymena thermophila size mutants: the relationship between cell size and regulation of DNA content. J Cell Sci 67:203–215Google Scholar
- Wacker M, Godard M (2005) Analysis of one-step and two-step real-time RT-PCR using SuperScript III. J Biomol Tech 16:266–271Google Scholar
- Wallbank RWR, Baxter SW, Pardo-Diaz C, Hanly JJ, Martin SH, Mallet J, Dasmahapatra KK, Salazar C, Joron M, Nadeau N, McMillan WO, Jiggins CD (2016) Evolutionary novelty in a butterfly wing pattern through Enhancer shuffling. PLoS Biol 14:e1002353. doi:10.1371/journal.pbio.1002353CrossRefGoogle Scholar