Expression of stress response HSP70 gene in Asian paddle crabs, Charybdis japonica, exposure to endocrine disrupting chemicals, bisphenol A (BPA) and 4-nonylphenol (NP)
- 671 Downloads
The Asian paddle crab, Charybdis japonica, is a potential bio-indicator reflecting marine sediment toxicity as well as a commercially important species living along coastal areas in Korea. This study investigated its stress response by looking at the heat shock protein (HSP70) gene of C. japonica when the organism is exposed to bisphenol A (BPA) and 4-nonylphenol (NP). We characterized partial sequence of HSP70 as the stressresponse gene of C. japonica. The nucleotide sequence of C. japonica HSP70 is over 90% homologous with the corresponding gene of other crabs. Phylogenetic tree analysis revealed a close relationship between C. japonica HSP70 and HSP70 in other species of lobster and shrimps. HSP70 mRNA transcripts were detected in all the examined tissues of C. japonica, with the highest level in gills, the organ that most frequently came into contact with the external BPA or NP-laden water. As no reference data were available for C. japonica crab exposure, the BPA and NP 24-h LC50 values have not been previously determined. The expression of the C. japonica HSP70 gene to various BPA or NP concentrations during short and longer times was assessed. Gene expression was significantly induced in concentration- and time-dependent manners after BPA or NP exposures. These results support the postulation that crab C. japonica HSP70 could be a potential stress response molecular marker to monitor marine ecosystems.
Key wordsCharybdis japonica bisphenol A 4-nonylphenol heat shock protein 70 ecosystem monitoring
Unable to display preview. Download preview PDF.
- Brooke LT, Thursby G (2005) Aquatic life ambient water quality criteria — nonylphenol. US Environmental Protection Agency (EPA), Office of Water Office of Science and Technology, Washington DC, 88 pGoogle Scholar
- Huang ZR, Sun DR, Chen ZZ, Zhang HH, Wang XH, Wang YZ, Fang HD, Dong YH (2009) Faunal characteristics and distribution pattern of crustaceans in the vicinity of Pearl River estuary. Ying Yong Sheng Tai Xue Bao 20:2535–2544Google Scholar
- Maguire RJ (1999) Review of the persistence of nonylphenol and nonylphenol ethoxylates in aquatic environments. Water Qual Res J Can 34:37–78Google Scholar
- Martin JW, Davis GE (2001) An updated classification of the recent crustacea. Natural History Museum of Los Angeles County, Los Angeles, 124 pGoogle Scholar
- Park K, Park J, Kim J, Kwak I-S (2010) Biological and molecular responses of Chironomus riparius (Diptera, Chironomidae) to herbicide 2,4-D (2,4-dichlorophenoxyacetic acid). Comp Biochem Phys C 151:439–446Google Scholar
- Pan L, Zhang H (2006) Metallothionein, antioxidant enzymes and DNA strand breaks as biomarkers of Cd exposure in a marine crab, Charybdis japonica. Comp Biochem Phys C 144:67–75Google Scholar
- Wee DP, Ng PKL (1995) Swimming crabs of the genera Charybdis De Haan, 1833 and Thalamita Latreille, 1829 (Crustacea: Decapoda: Brachyura: Portunidae) from Peninsular Malaysia and Singapore. Raffles Bull Zool 1:1–128Google Scholar
- Wu R, Sun Y, Lei LM, Xie ST (2008) Molecular identification and expression of heat shock cognate 70 (HSC70) in the pacific white shrimp Litopenaeus vannamei. Mol Biol 42:265–274Google Scholar
- Zhang XY, Zhang MZ, Zheng CJ, Liu J, Hu HJ (2009) Identification of two hsp90 genes from the marine crab, Portunus trituberculatus and their specific expression profiles under different environmental conditions. Comp Biochem Phys C 150:465–473Google Scholar