Phytoparasitica

, Volume 41, Issue 5, pp 557–568 | Cite as

Characterization of Phytophthora infestans populations of southern Brazil in 2004 and 2005

  • Flávio Martins Santana
  • Cesar Bauer Gomes
  • Cesar Rombaldi
  • Valmor João Bianchi
  • Ailton Reis
Article
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Abstract

The populations of Phytophthora infestans (Pi) in southern Brazil in 2004 and 2005 are characterized herein. The isolates were collected from potato and tomato plants in the states of Paraná (PR), Santa Catarina (SC), and Rio Grande do Sul (RS). The mating type of 131 potato and 32 tomato isolates was determined. Forty-nine isolates from potatoes and 11 from tomatoes were analyzed for their Gpi phenotype. A subset of 35 isolates was evaluated for mitochondrial (mtDNA) polymorphisms. A sample of 146 isolates was tested for sensitivity to the fungicide metalaxyl, and most isolates (64%) were moderately sensitive. Fifty-nine isolates were classified as A1 mating type and 103 as A2. One isolate behaved as both A1 and A2 mating type. All tomato isolates were A1 mating type and presented the 86/100 pattern for the enzyme GPI and mtDNA Ib, indicating that these isolates belong to the US-1 clonal lineage. Of the 131 potato isolates, 103 were A2, 27 were A1 and one was A1/A2 mating type. Among the potato isolates 27 exhibited the Gpi phenotype 100/100, the same as BR-1, and 20 were 86/100, the same as US-1. Potato isolates presented the mitochondrial haplotypes Ia (74%) and IIa (26%). The data suggest the presence of only the BR-1 clonal lineage on potatoes in the states of PR and SC. However, in the state of RS, more than one clonal lineage was observed infecting potatoes, and there may be sexual reproduction between the lineages.

Keywords

Late blight Pathogen characterization Population genetics Solanum lycopersicum Solanum tuberosum 
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Notes

Acknowledgment

The authors thank CNPq (Brazilian Council for Scientific Research) for the partial funding of this work and for the Ailton Reis research fellowship.

References

  1. Adler, N. E., Erselius, L. J., Chacón, M. G., Flier, W. G., Ordoñez, M. E., Kroon, L. P. N. M., et al. (2004). Genetic diversity of Phytophthora infestans sensu lato in Ecuador provides new insight into the origin of this important plant pathogen. Phytopathology, 94, 154–162.PubMedCrossRefGoogle Scholar
  2. Carter, D. A., Archer, S. A., Buck, K. W., Shaw, D. S., & Shattock, R. C. (1990). Restriction fragment length polymorphisms of mitochondrial DNA of Phytophthora infestans. Mycological Research, 94, 1123–1128.CrossRefGoogle Scholar
  3. Cooke, D. E., Cano, L. M., Raffaele, S., Bain, R. A., Cooke, L. R., Etherington, G. J., et al. (2012). Genome analyses of an aggressive and invasive lineage of the Irish potato famine pathogen. PLOS Pathogens, 8, e1002940. www.plospathogens.org.PubMedCrossRefGoogle Scholar
  4. Cooke, D. E. L., & Lees, A. K. (2004). Markers, old and new, for examining Phytophthora infestans diversity. Plant Pathology, 53, 692–704.CrossRefGoogle Scholar
  5. Deahl, K. L., Inglis, D. A., & Demuth, S. P. (1993). Testing for resistance to metalaxyl in Phytophthora infestans isolates from northwestern Washington. American Potato Journal, 70, 779–795.CrossRefGoogle Scholar
  6. Deahl, K. L., Pagani, M. C., Vilaro, F. L., Perez, F. M., Moravec, B., & Cooke, L. R. (2003). Characteristics of Phytophthora infestans isolates from Uruguay. European Journal of Plant Pathology, 109, 277–281.CrossRefGoogle Scholar
  7. Forbes, G. A., Escobar, X. C., Ayala, C. C., Revelo, J., Ordóñez, M. E., Fry, B. A., et al. (1997). Population genetic structure of Phytophthora infestans in Ecuador. Phytopathology, 87, 375–380.PubMedCrossRefGoogle Scholar
  8. Forbes, G. A., Goodwin, S. B., Drenth, A., Oyarzun, P., Ordoñez, M. E., & Fry, W. E. (1998). A global marker database for Phytophthora infestans. Plant Disease, 82, 811–818.CrossRefGoogle Scholar
  9. Fry, W. E., & Goodwin, S. B. (1997). Re-emergence of potato and tomato late blight in the United States. Plant Disease, 87, 1349–1357.CrossRefGoogle Scholar
  10. Fry, W. E., Goodwin, S. B., Matuszak, J. N., Spielman, L. J., Milgroom, M. G., & Drenth, A. (1992). Population genetics and intercontinental migrations of Phytophthora infestans. Annual Review of Phytopathology, 30, 107–129.CrossRefGoogle Scholar
  11. Gallegly, M. E., & Galindo, J. (1958). Mating types and oospores of Phytophthora infestans in nature in Mexico. Phytopathology, 48, 274–277.Google Scholar
  12. Garry, G., Forbes, G. A., Salas, A., Santa Cruz, M., Perez, W. G., & Nelson, R. J. (2005). Genetic diversity and host differentiation among isolates of Phytophthora infestans from cultivated potato and wild solanaceous hosts in Peru. Plant Pathology, 54, 740–748.CrossRefGoogle Scholar
  13. Gavino, P. D., & Fry, W. E. (2002). Diversity in and evidence for selection on the mitochondrial genome of Phytophthora infestans. Mycologia, 94, 781–793.PubMedCrossRefGoogle Scholar
  14. Gavino, P. D., Smart, C. D., Sandrock, R. W., Miller, J. S., Hamm, P. B., Lee, T. Y., et al. (2000). Implications of sexual reproduction for Phytophthora infestans in the United States: Generation of an aggressive lineage. Plant Disease, 84, 731–735.CrossRefGoogle Scholar
  15. Goodwin, S. B. (1997). The population genetics of Phytophthora. Phytopathology, 87, 462–473.PubMedCrossRefGoogle Scholar
  16. Goodwin, S. B., Cohen, B. A., & Fry, W. E. (1994). Panglobal distribution of a single clonal lineage of the Irish potato famine fungus. Proceedings of the National Academy of Sciences USA, 91, 11591–11595.CrossRefGoogle Scholar
  17. Griffith, G. W., & Shaw, D. (1998). Polymorphisms in Phytophthora infestans: four mitochondrial haplotypes are detected after PCR amplification of DNA from pure cultures or from host lesions. Applied and Environmental Microbiology, 64, 4007–4014.PubMedGoogle Scholar
  18. Hohl, H. R., & Iselin, K. (1984). Strains of Phytophthora infestans from Switzerland with A2 mating type behaviour. Transactions of the British Mycological Society, 83, 529–531.CrossRefGoogle Scholar
  19. Jones, J. B., Jones, J. P., Stall, R. E., & Zitter, T. A. (1991). Compendium of tomato diseases. St. Paul, MN, USA: APS Press.Google Scholar
  20. Judelson, H. S. (1996). Chromosomal heteromorphism linked to the mating type locus of the oomycete Phytophthora infestans. Molecular General Genetics, 252, 155–161.PubMedCrossRefGoogle Scholar
  21. Mizubuti, E. S. G. (2001). Requeima ou mela da batata e do tomate. In E. D. N. Luz, A. F. Santos, K. Matsuoka, & J. L. Bezerra (Eds.), Doenças causadas por Phytophthora no Brasil (pp. 100–174). Campinas, SP, Brazil: Editora Rural.Google Scholar
  22. Reis, A., Ribeiro, F. H. S., Maffia, L. A., & Mizubuti, E. S. G. (2005). Sensitivity of Brazilian isolates of Phytophthora infestans to commonly used fungicides in tomato and potato crops. Plant Disease, 89, 1279–1284.CrossRefGoogle Scholar
  23. Reis, A., Ribeiro, F. H. S., & Mizubuti, E. S. G. (2006). Caracterização de isolados de Phytophthora infestans do Distrito Federal e de Goiás. Fitopatologia Brasileira, 31, 270–276.CrossRefGoogle Scholar
  24. Reis, A., Smart, C. D., Fry, W. E., Maffia, L. A., & Mizubuti, E. S. G. (2003). Characterization of isolates of Phytophthora infestans from southern and southeastern regions of Brazil, from 1998 to 2001. Plant Disease, 87, 896–900.CrossRefGoogle Scholar
  25. Ristaino, J. B. (2002). Tracking historic migrations of the Irish potato famine pathogen Phytophthora infestans. Microbes and Infection, 4, 1369–1377.PubMedCrossRefGoogle Scholar
  26. Ristaino, J. B., Hu, C. H., & Fitt, B. D. L. (2012). Evidence for presence of the founder Ia mtDNA haplotype of Phytophthora infestans in 19th century potato tubers from the Rothamsted archives. Plant Pathology, 61, 1–9.CrossRefGoogle Scholar
  27. Stevenson, W. R., Loria, R., Franc, G. D., & Weingartner, D. P. (2001). Compendium of potato diseases. St. Paul, MN, USA: APS Press.Google Scholar
  28. Suassuna, N. D., Maffia, L. A., & Mizubuti, E. S. G. (2004). Aggressiveness and host specificity of Brazilian isolates of Phytophthora infestans. Plant Pathology, 53, 405–413.CrossRefGoogle Scholar
  29. Therrien, C. D., Tooley, P. W., Spielman, L. J., Fry, W. E., Ritch, D. L., & Shelly, S. E. (1993). Nuclear DNA content, allozyme phenotypes and metalaxyl sensitivity of Phytophthora infestans from Japan. Mycological Research, 97, 945–950.CrossRefGoogle Scholar
  30. Vargas, A. M., Quesada Ocampo, L. M., Céspedes, M. C., Carreño, N., González, A., Rojas, A., et al. (2009). Characterization of Phytophthora infestans populations in Colombia: First report of the A2 mating type. Phytopathology, 99, 82–88.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Flávio Martins Santana
    • 1
    • 2
  • Cesar Bauer Gomes
    • 3
  • Cesar Rombaldi
    • 4
  • Valmor João Bianchi
    • 5
  • Ailton Reis
    • 6
  1. 1.PPG Fitossanidade, Universidade Federal de Pelotas (UFPel)PelotasBrazil
  2. 2.Laboratório de Fitopatologia, Embrapa TrigoPasso FundoBrazil
  3. 3.Laboratorio de Fitopatologia, Embrapa Clima TemperadoPelotasBrazil
  4. 4.Laboratório de Tecnologia de Alimentos, UFPelPelotasBrazil
  5. 5.Laboratório de Botânica, UFPelPelotasBrazil
  6. 6.Embrapa HortaliçasBrasiliaBrazil

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