, Volume 39, Issue 5, pp 455–460 | Cite as

Activity of ß-1,3-glucanase and ß-1,4-glucanase in two potato cultivars following challenge by the fungal pathogen Alternaria solani

  • Hadis Shahbazi
  • Heshmatolah Aminian
  • Navazollah Sahebani
  • Dennis A. Halterman


Early blight of potato, caused by Alternaria solani, is a ubiquitous disease in many countries around the world. Our previous screening of several Iranian potato cultivars found that variation in resistance exists between two cultivars: ‘Diamond’ and ‘Granula’. Cultivar Diamond is more resistant to multiple isolates of A. solani when compared to cv. Granula. Furthermore, we have found that different pathogen isolates have varying degrees of infection. We monitored the activities of two pathogen-related (PR) glucanase proteins in Diamond and Granula in response to two isolates of A. solani with different degrees of virulence. ß-1,3-glucanase and ß-1,4-glucanase activities were recorded in healthy and diseased leaves of potatoes up to 10 days after inoculation. Their activities were found to be higher in diseased leaves when compared to those of uninfected leaves. Our data suggest that significantly reduced activities of theses enzymes in potato could be related to a lower degree of resistance or an increased ability of a more aggressive isolate to suppress PR protein expression.


Early blight Pathogen-related protein Solanum tuberosum 



The authors are grateful to Dr. H. R. Etebarian (Department of Plant Protection, Aboryhan Campus, Tehran University, Tehran, Iran) for helpful suggestions. The authors also gratefully acknowledge the valuable support of Mr. M. R. Lak in the study.


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Copyright information

© Springer Science + Business Media B.V. 2011

Authors and Affiliations

  • Hadis Shahbazi
    • 1
  • Heshmatolah Aminian
    • 2
  • Navazollah Sahebani
    • 2
  • Dennis A. Halterman
    • 3
  1. 1.Young Researchers Club, Arak BranchIslamic Azad UniversityArakIran
  2. 2.Department of Plant Protection, Aboryhan CampusTehran UniversityTehranIran
  3. 3.U.S. Department of AgricultureAgricultural Research Service, Vegetable Crops Research UnitMadisonUSA

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