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A molecular mechanism of resistance to streptomycin in Xanthomonas oryzae pv. oryzicola

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Abstract

Xanthomonas oryzae pv. oryzicola, the causal agent of rice leaf streak disease, was found to be sensitive to streptomycin (an aminocyclitol glycoside antibiotic), by inhibition of protein synthesis resulting from interference with translational proofreading. This study aimed to determine the molecular resistance mechanism of X. oryzae pv. oryzicola to streptomycin. Seven streptomycin-resistant mutants were obtained by UV induction or streptomycin selection. These mutants can grow at 100 μg ml−1 of streptomycin while the wild-type strain (RS105) cannot grow at 5 μg ml−1. Sequencing indicated that the rpsL gene encoding ribosomal protein S12 has 375 bp encoding 125 amino acid residues. In all resistant strains, a mutation in which AAG was substituted for AGG (Lys→Arg) occurred either at codon 43 or 88. Two plasmids, pUFRRS and pUFRRX, were constructed by ligating the rpsL gene into the cosmid pUFR034. The plasmids pUFRRS and pUFRRX containing the Lys→Arg mutation of the rpsL gene conferred streptomycin resistance to the sensitive wild-type strain by electroporation. Both transformants, RS1 and RS2, could grow in the medium containing 50 μg ml−1 of streptomycin. A mutation at codon 43 or 88 in rpsL can result in resistance of Xanthomonas oryzae pv. oryzicola to streptomycin.

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Acknowledgments

This study was sponsored by the State “973” Programs from the Ministry of Science and Technology of China (2009CB118906 & 2006CB101907), the State “863” Programs from the Ministry of Science and Technology of China (2008AA10Z414), the National Natural Science Foundation of China (30800730), the program for public welfare industry from the Ministry of Agriculture of China (3–32:) and Anhui Provincial Natural Science Foundation (10040606Q26).

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Authors and Affiliations

  1. College of Crop Protection, Nanjing Agricultural University, Nanjing, 210095, China

    Yong Zhang, Xiaofeng Zhu, Ying Xu, Yiping Hou & Mingguo Zhou

  2. Institute of Plant Protection, Anhui Academy of Agricultural Sciences, Hefei, 230031, China

    Yong Zhang, Yu Chen & Tongchun Gao

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  1. Yong Zhang
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  2. Yu Chen
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  3. Xiaofeng Zhu
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  4. Ying Xu
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  5. Yiping Hou
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  6. Tongchun Gao
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  7. Mingguo Zhou
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Correspondence to Mingguo Zhou.

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Yong Zhang and Yu Chen share joint first authorship

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Zhang, Y., Chen, Y., Zhu, X. et al. A molecular mechanism of resistance to streptomycin in Xanthomonas oryzae pv. oryzicola . Phytoparasitica 39, 393–401 (2011). https://doi.org/10.1007/s12600-011-0172-6

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  • DOI: https://doi.org/10.1007/s12600-011-0172-6

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