, Volume 39, Issue 4, pp 325–331 | Cite as

Effect of Beauveria bassiana mixed with diatomaceous earth on mortality, mycosis and sporulation of Rhyzopertha dominica on stored wheat

  • Tahira Riasat
  • Waqas Wakil
  • Muhammad Ashfaq
  • Shahbaz Talib Sahi


A laboratory assay was designed to determine the insecticidal efficacy of Beauveria bassiana (Balsamo) Vuillemin (Hyphomycetes: Moniliales) and diatomaceous earth (Diafil 610) against Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae). The fungus B. bassiana was applied at 2.23 × 107, 2.23 × 108 and 2.23 × 109 conidia kg−1 of wheat individually as well as mixed with 200 and 400 ppm of Diafil 610. The conditions for the trials were 30 ± 2oC with 55% r.h. and the counts for mortality were made after 8, 16 and 24 d. All the dead adults were removed after each count and the vials were kept for the next 60 d to assess the emergence of the F1 generation. The findings from these studies proved that the extended exposure interval and the highest combined dose rate of the entomopathogenic fungus and the diatomaceous earth gave the maximum mortality of the beetles. The emergence of the progeny was also highly suppressed where the maximum dose rate of the synergized treatments was applied. The rate of mycosis and sporulation in the cadavers of R. dominica was maximum where the low dose rates of B. bassiana were applied.


Desiccant dust Entomopathogenic fungi Lesser grain borer Stored grain 



This research work was supported by a scholarship from Higher Education Commission, Islamabad, Pakistan (074-2407-Av4-128) under Indigenous Ph.D. Fellowship Program.


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Copyright information

© Springer Science & Business Media BV 2011

Authors and Affiliations

  • Tahira Riasat
    • 1
  • Waqas Wakil
    • 1
  • Muhammad Ashfaq
    • 1
  • Shahbaz Talib Sahi
    • 2
  1. 1.Department of Agricultural EntomologyUniversity of AgricultureFaisalabadPakistan
  2. 2.Department of Plant PathologyUniversity of AgricultureFaisalabadPakistan

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