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Resistance to chlorpyrifos and lambda-cyhalothrin along with detoxifying enzyme activities in field-collected female populations of European red mite

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Abstract

The levels of susceptibility of populations of the European red mite Panonychus ulmi (Koch) (Acarina: Tetranychidae) collected from apple orchards in the Bursa region of Turkey to the insecticides chlorpyrifos and lambda-cyhalothrin, were determined by a petri leaf disk—Potter spray tower method. When compared with the susceptible population, resistance ratios at the LC50 level ranged from 6.0- to 35.6-fold, and from 0.7- to 5.7-fold for chlorpyrifos and lambda-cyhalothrin, respectively. Kinetic parameters of general esterase activity with α-naphthyl acetate as substrate indicated that an increased activity was present in the resistant populations compared with the susceptible populations. In these strains, 1.5- and 2.2-fold higher Glutathione S-transferase (GST) activity was also detected with the substrate 1-chloro-2,4-dinitrobenzene. General esterase activity gel profiles of these populations were studied by native polyacrylamide gel electrophoresis, but no relationship between resistance ratios and band patterns was detected. The results of this study document a decreased efficacy of chlorpyrifos and lambda-cyhalothrin in field populations of P. ulmi in Turkey, possibly linked to altered activities of esterases and GST.

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Acknowledgments

We thank the Scientific and Technical Research Council of Turkey–TUBITAK (Projects Grant No. TOVAG-107 O 286) for financial assistance.

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Authors and Affiliations

  1. Plant Protection Department, Faculty of Agriculture, Uludag University, 16059, Gorukle, Bursa, Turkey

    Nabi Alper Kumral & Nimet Sema Gençer

  2. Bursa Province Directorate of Ministry of Agriculture and Rural Affairs, 16170, Hürriyet, Bursa, Turkey

    Hilal Susurluk

  3. Plant Protection Department, Faculty of Agriculture, Ankara University, 06110, Dışkapı, Turkey

    Mehmet Oktay Gürkan

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  1. Nabi Alper Kumral
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  2. Hilal Susurluk
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Correspondence to Nabi Alper Kumral.

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Kumral, N.A., Susurluk, H., Gençer, N.S. et al. Resistance to chlorpyrifos and lambda-cyhalothrin along with detoxifying enzyme activities in field-collected female populations of European red mite. Phytoparasitica 37, 7–15 (2009). https://doi.org/10.1007/s12600-008-0007-2

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