Flowing cells stability test and evaluation for fast flow cytometry
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Cell sorting requires a stable flowing speed, especially for a fast flow cytometry like the optical time-stretch imaging. The flowing speed fluctuation of Euglena cells and Nile red particles is tested and evaluated by a home-built flowing speed testing system. Higher flowing speeds have better stability. Flowing microchannels with controlling steps also improve the speed stability. The time fluctuation is ~ 1 ms under a flowing speed of ~ 1.4 m/s. Cell sorting can be realized by either using an ultra-short delay time between the sorting point and imaging point, which is suitable for relative lower cells counting speed (~ 5000 cells/s), or developing effective fluctuation controlling methods for higher counting speed, like using step glass microchannels.
KeywordsCytometry Cell sorting Flowing speed test Flowing cells stability
This study was supported by National Natural Science Foundation of China (NSFC) under Grant 61605140.
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