Effects of Fruit Ripening Retardant Alar (Daminozide) on Behaviour of Drosophila melanogaster
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Alar (Daminozide), a plant growth retardant, is used in different fruit orchards especially of apples and mangoes to increase the production by preventing pre harvest fruit drop, promoting colour development and increasing the storage life. In an earlier study we have demonstrated both neurotoxic and teratogenic effects of Alar in a model organism Drosophila melanogaster. As an extension of the previous work, the present study is designed to investigate the effects of Alar on larval and adult behaviour of the fly. The Results demonstrated no significant alteration in feeding rate of treated larvae, but their foraging path length was shortened considerably, indicating impaired locomotion. Moreover, the onset of their specific courtship behavior like orientation, tapping and wing vibration was significantly delayed which suggest that the treated males were less vigorous and females were less receptive than the controls. In addition, Alar treatment was associated with reduction in copulation duration and subsequent decline in Courtship Index indicating lower reproductive fitness. Collectively, the findings suggest that Alar is ethotoxic for D. melanogaster.
KeywordsAlar Drosophila melanogaster Behaviour Feeding rate Foraging path length Courtship assay
The work was carried with the financial and instrumental support from UGC-UPE Phase II, DST-FIST and PURSE. We are thankful to the Head, Department of Zoology for providing necessary logistic supports.
Compliance with ethical standards
Conflict of interest
- Algarve, T.D., C.E. Assmann, T. Aigaki, and I.B.M. da Cruz. 2018. Parental and preimaginal exposure to methylmercury disrupts locomotor activity and circadian rhythm of adult Drosophila melanogaster. Drug and Chemical Toxicology. https://doi.org/10.1080/01480545.2018.1485689.CrossRefPubMedGoogle Scholar
- Ashburner, M., K.G. Golic, and R.S. Hawley. 2005. Drosophila: A laboratory handbook, 2nd ed. New York, USA: Cold Spring Harbor Laboratory Press.Google Scholar
- Ejima, A., and L.C. Griffith. 2007. Measurement of courtship behavior in Drosophila melanogaster, 4847. New York: Cold Spring Harbor protocols.Google Scholar
- Figueira, F.H., N. de Quadros Oliveira, L.M. de Aguiar, A.L. Escarrone, E.G. Primel, D.M. Barros, and C.E. da Rosa. 2017. Exposure to atrazine alters behaviour and disrupts the dopaminergic system in Drosophila melanogaster. Comparative Biochemistry and Physiology- Part C: Toxicology & Pharmacology 202: 94–102.Google Scholar
- Gordon, W. 2011. The true Alar story: Part I. http://www.onearth.org/blog/the-true-alar-story.
- Guruprasad, B.R., S.N. Hegde, and M.S. Krishna. 2008. Positive correlation between male size and remating success in few populations of D. bipectinata. Zoological Studies 47: 75–83.Google Scholar
- Leão, M.B., P.C.C. da Rosa, C. Wagner, T.H. Lugokenski, and C.L. Dalla Corte. 2018. Methylmercury and diphenyl diselenide interactions in Drosophila melanogaster: Effects on development, behavior, and Hg levels. Environmental Science and Pollution Research International 25: 21568–21576.CrossRefGoogle Scholar
- Nazari, M., and S.N. Hegde. 2006. Effect of flouxetine on the courtship latency, mating latency and copulation duration of Drosophila melanogaster. Journal of Postgraduate Medical Institute 20: 58–63.Google Scholar
- Nichols, C.D., J. Becnel, and U.B. Pandey. 2012. Methods to assay Drosophila behavior. Journal of Visualized Experiments 61: e3795.Google Scholar