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Effect of cypermethrin on worker and soldier termites of subterranean termites Odontotermes brunneus (Hagen) (Termitidae: Isoptera)

  • Venkatesulu Mamatha
  • Ranganathan MuthusamyEmail author
  • Jimmantiyur Madhappan Murugan
  • Eliningaya J. Kweka
Research Article
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Abstract

The termite Odontotermes brunneus is an economically important species causing damage to cellulose containing wooden material and agricultural crops in India. Insecticide application is an effective strategy in termite control. In the present study the effect of cypermethrin was tested for workers and soldiers termite using filter paper dip method. After 24 h treatment the lethal concentration (LC50) was increased to 9.7 ppm in workers and 1.8 ppm in soldiers respectively. The detoxification enzyme activities of esterase, glutathione S-transferase was increased in worker termites 23 µmol, 9 µmol/min/mg of protein compared to soldiers 15 µmol, 7 µmol/min/mg of protein respectively (p < 0.05). The activity of mixed-function oxidase was found very less in both samples. Further nPAGE analysis revealed that increased esterase band in workers than soldier and control sample. The data of this study revealed that possible mechanism of esterase and glutathione S-transferase mediated cypermethrin detoxification that leads to reduce the sensitivity in worker termites of O. brunneus.

Keywords

Terrestrial insect Synthetic pyrethriod Toxicity Detoxification enzymes Electrophoresis 

Notes

Acknowledgements

We thank our department of PG and Research Centre in Biotechnology for providing infrastructure facility to carry out this work.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Zoological Society, Kolkata, India 2019

Authors and Affiliations

  • Venkatesulu Mamatha
    • 1
  • Ranganathan Muthusamy
    • 1
    Email author
  • Jimmantiyur Madhappan Murugan
    • 1
  • Eliningaya J. Kweka
    • 2
    • 3
  1. 1.PG and Research Centre in BiotechnologyMGR CollegeHosurIndia
  2. 2.Department of Medical Parasitology and Entomology, School of MedicineCatholic University of Health and Allied SciencesMwanzaTanzania
  3. 3.Mosquito Section, Division of Livestock and Human Health Disease Vector ControlTropical Pesticides Research InstituteArushaTanzania

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