Isolation of Fluoride Tolerant Bacillus spp (KT201599, KT201600) from the Midgut of Drosophila melanogaster: Their Probable Role in Fluoride Removal
This is for the first time that two fluoride tolerant bacterial strains of Bacillus spp (KT201599 and KT201600) have been isolated from the mid-gut of third instar larvae of Drosophila melanogaster. They are characterized and identified using phenotypic characters and 16S rRNA gene based molecular phylogenetic analysis. Interestingly, KT201599 and KT201600 are able to survive at 2000–2500 µg/mL sodium fluoride (NaF) concentrations. The chronic lethal concentration 50 (LC50) for NaF in Drosophila larvae is 125 µg/mL, whereas, the above-mentioned Bacillus strains isolated from their mid-gut are found to be more tolerant to fluoride (F) in comparison to their host. In search for biological relevance of KT201599 and KT201600, we further deciphered that these strains not only survived at high F concentrations, but also were efficient in removing the fluoride ions from the medium by 16.66 and 24.71% respectively. Thus, the study indicates that resident gut flora of Drosophila having the property of reducing fluoride load from the medium, might be helping the host to thrive in a fluoridated environment.
KeywordsBacteria Bacillus Bioremediation Drosophila melanogaster Fluoride
The authors are grateful to the Head, DST-FIST and UGC-DRS sponsored Department of Zoology, the University of Burdwan for providing the infrastructural facilities. Thanks are due to Prof. Abhijit Mazumdar and Dr. Niladri Hazra and Dr. S Chakraborty, USIC (University Science Instrumentation Centre for kindly providing microscopic facilities and BOD incubator for bacterial growth. Authors are grateful to Prof. Apurba Ratan Ghosh and Dr. Shrimanta Gupta, Dept of Environmental Science, The University of Burdwan for their kind help during fluoride estimation.
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