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The Journal of Physiological Sciences

, Volume 69, Issue 3, pp 531–541 | Cite as

Effects of acute kidney dysfunction on hypothalamic arginine vasopressin synthesis in transgenic rats

  • Hiromichi Ueno
  • Ryota Serino
  • Kenya Sanada
  • Yasuki Akiyama
  • Kentaro Tanaka
  • Haruki Nishimura
  • Kazuaki Nishimura
  • Satomi Sonoda
  • Yasuhito Motojima
  • Reiko Saito
  • Mitsuhiro Yoshimura
  • Takashi Maruyama
  • Tetsu Miyamoto
  • Masahito Tamura
  • Yutaka Otsuji
  • Yoichi UetaEmail author
Original Paper

Abstract

Acute loss of kidney function is a critical internal stressor. Arginine vasopressin (AVP) present in the parvocellular division of the paraventricular nucleus (PVN) plays a key role in the regulation of stress responses. However, hypothalamic AVP dynamics during acute kidney dysfunction remain unclear. In this study, we investigated the effects of bilateral nephrectomy on AVP, using a transgenic rat line that expressed the AVP-enhanced green fluorescent protein (eGFP). The eGFP fluorescent intensities in the PVN were dramatically increased after bilateral nephrectomy. The mRNA levels of eGFP, AVP, and corticotrophin-releasing hormone in the PVN were dramatically increased after bilateral nephrectomy. Bilateral nephrectomy also increased the levels of Fos-like immunoreactive cells in brainstem neurons. These results indicate that bilateral nephrectomy upregulates the AVP-eGFP synthesis. Further studies are needed to identify the neural and/or humoral factors that activate AVP synthesis and regulate neuronal circuits during acute kidney dysfunction.

Keywords

Vasopressin Transgenic rat In situ hybridization Hypothalamus Bilateral nephrectomy 

Notes

Acknowledgements

We express our appreciation to Ms. Yuki Nonaka for her technical assistance.

Author contributions

Conception or design of the work; HU, RS, TM, MT, YO, YU. Acquisition, analysis, or interpretation of data for the work; HU, KS, YA, KT, HN, KN, SS, YM, RS, MY, TM. Drafting the work or revising it critically for important intellectual content; HU, RS, KS, YA, KT, HN, KN, SS, YM, RS, MY, TM, MT, YO, YU. All authors approved the final version of the manuscript and agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated.

Funding

This paper was supported by a Grant-in-Aid for Scientific Research (B) (No. 17H04027), (C) (No. 17K08582), and Young Scientist (B) (No. 17K15575) from the Japan Society for the Promotion of Science (JSPS).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures in this study were performed in accordance with the guidelines on the use and care of the laboratory animals as set out care by the Physiological Society of Japan and under control of the Ethics Committee of Animal Care and Experimentation, University of Occupational and Environmental Health (Fukuoka, Japan).

Supplementary material

12576_2019_675_MOESM1_ESM.pptx (55 kb)
Supplementary material 1 (PPTX 54 kb)

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Copyright information

© The Physiological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Hiromichi Ueno
    • 1
    • 2
  • Ryota Serino
    • 3
  • Kenya Sanada
    • 1
    • 2
  • Yasuki Akiyama
    • 1
  • Kentaro Tanaka
    • 1
  • Haruki Nishimura
    • 1
  • Kazuaki Nishimura
    • 1
  • Satomi Sonoda
    • 1
  • Yasuhito Motojima
    • 1
  • Reiko Saito
    • 1
  • Mitsuhiro Yoshimura
    • 1
  • Takashi Maruyama
    • 1
  • Tetsu Miyamoto
    • 2
  • Masahito Tamura
    • 2
  • Yutaka Otsuji
    • 2
  • Yoichi Ueta
    • 1
    Email author
  1. 1.Department of Physiology, School of MedicineUniversity of Occupational and Environmental HealthKitakyushuJapan
  2. 2.The Second Department of Internal Medicine, School of MedicineUniversity of Occupational and Environmental HealthKitakyushuJapan
  3. 3.Department of NephrologyYoshino HospitalKitakyushuJapan

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