Exhaustive exercise decreases renal organic anion transporter 3 function
- 79 Downloads
This study aimed to investigate the effects of various types of exercise on organic anion transporter 3 (Oat3) function, a major transporter that plays a role in the secretion of a variety of drugs and endogenous compounds. Male Wistar rats were randomly allocated to non-exercise, exhaustive, acute and training exercise groups. The function of Oat3 was assessed by the uptake of [3H]-estrone sulfate ([3H]-ES) into rat renal cortical slices. Acute and training exercises had no effect on [3H]-ES uptake whereas a marked reduction in [3H]-ES uptake occurred immediately after exhaustive exercise. However, the reduction in Oat3 function was gradually recovered at 6 and 24 h after the exercise session. Importantly, the impairment of Oat3 function was associated with a decrease in renal Oat3 protein expression. Our results indicate that exhaustive exercise produces a significant impact on renal organic anion transport function, which in turn could alter the plasma level of drugs and compounds in the body.
KeywordsExercise Exhaustive exercise Training Renal secretory process Organic anion transporter 3
The proof reading of this manuscript was supported by the Editorial Office, Faculty of Graduate Studies, Mahidol University.
TB, CY, and RN performed biochemical assays, uptake study, and animal experiment. TB analyzed data, interpreted results and wrote the manuscript. TB and VC designed the project. VC supervised the project and revised the manuscript.
This study was supported by the Thailand Research Fund (TRG5780104) and Mahidol University (TM 42/2557).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures in this study were conducted in accordance with the guidelines of the National Laboratory Animal Center of Thailand. The protocol was approved by the Animal Care and Use Committee of the Faculty of Science, Mahidol University, Thailand (MUSC57-003-298).
- 1.American College of Sports Medicine (2014) ACSM’s guidelines for exercise testing and prescription. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
- 2.American College of Sports Medicine (2012) ACSM’s advanced exercise physiology. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
- 6.Somani SM, Kamimori GH (1996) Pharmacology in exercise and sports. CRC Press, Boca RatonGoogle Scholar
- 7.Ylitalo P, Hinkka H (1985) Effect of exercise on plasma levels and urinary excretion of sulphadimidine and procainamide. Int J Clin Pharmacol Ther Toxicol 23:548–553Google Scholar
- 13.Motohashi H, Sakurai Y, Saito H, Masuda S, Urakami Y, Goto M, Fukatsu A, Ogawa O, Inui K (2002) Gene expression levels and immunolocalization of organic ion transporters in the human kidney. J Am Soc Nephrol 13:866–874Google Scholar
- 15.Sakurai Y, Motohashi H, Ueo H, Masuda S, Saito H, Okuda M, Mori N, Matsuura M, Doi T, Fukatsu A, Ogawa O, Inui K (2004) Expression levels of renal organic anion transporters (OATs) and their correlation with anionic drug excretion in patients with renal diseases. Pharm Res 21:61–67CrossRefGoogle Scholar
- 20.Eaton DC, Pooler JP (2009) Vander’s renal physiology. McGraw-Hill Education, New YorkGoogle Scholar
- 21.Perrone RD, Madias NE, Levey AS (1992) Serum creatinine as an index of renal function: new insights into old concepts. Clin Chem 38:1933–1953Google Scholar
- 23.Mazzeo RS, Brooks GA, Horvath SM (1984) Effects of age on metabolic responses to endurance training in rats. J Appl Physiol Respir Environ Exerc Physiol 57:1369–1374Google Scholar
- 27.Schneider R, Sauvant C, Betz B, Otremba M, Fischer D, Holzinger H, Wanner C, Galle J, Gekle M (2007) Downregulation of organic anion transporters OAT1 and OAT3 correlates with impaired secretion of para-aminohippurate after ischemic acute renal failure in rats. Am J Physiol Renal Physiol 292:F1599–F1605CrossRefGoogle Scholar
- 29.Brooks GA, Fahey TD, Baldwin KM (2005) Exercise physiology: human bioenergetics and its applications. McGraw-Hill Education, BostonGoogle Scholar