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The Journal of Physiological Sciences

, Volume 69, Issue 1, pp 13–21 | Cite as

Isoflurane anesthesia does not affect spinal cord neurovascular coupling: evidence from decerebrated rats

  • Thierry Paquette
  • Hugues Leblond
  • Mathieu PichéEmail author
Original Paper

Abstract

Neurological examination remains the primary clinical investigation in patients with spinal cord injury. However, neuroimaging methods such as functional magnetic resonance imaging (fMRI) are promising tools for following functional changes in the course of injury, disease and rehabilitation. However, the relationship between neuronal activity and blood flow in the spinal cord on which fMRI relies has been largely overlooked. The objective of this study was to examine neurovascular coupling in the spinal cord of decerebrated rats during electrical stimulation of the sciatic nerve with and without isoflurane anesthesia (1.2%). Local field potentials (LFP) and spinal cord blood flow (SCBF) were recorded simultaneously in the lumbosacral enlargement. Isoflurane did not significantly alter LFP (p = 0.53) and SCBF (p = 0.57) amplitude. Accordingly, neurovascular coupling remained comparable with or without isoflurane anesthesia (p = 0.39). These results support the use of isoflurane in rodents to investigate nociceptive functions of the spinal cord using fMRI.

Keywords

Spinal cord Neurovascular coupling Pain Nociception Blood flow Local field potential 

Notes

Acknowledgements

This project was funded by the Natural Science and Engineering Research Council (NSERC) of Canada (Grant Numbers: 402176 (MP); 05403 (HL)). TP was supported by scholarships from the NSERC, the department of anatomy (UQTR) and the “Fonds de Recherche du Québec en Santé” (FRQS). The contribution of Mathieu Piché was supported by the UQTR research chair in pain neurophysiology, the “Fondation de Recherche en Chiropratique du Québec” and the FRQS.

Author contribution

TP contributed to all aspects of the research. HL contributed to all aspects of the research. MP contributed to all aspects of the research and obtained funding for the study.

Funding

This study was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (Grant Numbers: 402176 (MP); 05403 (HL)).

Compliance with ethical standards

Conflict of interest

Thierry Paquette reports no financial or other relationship that may lead to any conflict of interest. Hugues Leblond reports no financial or other relationship that may lead to any conflict of interest. Mathieu Piché reports no financial or other relationship that may lead to any conflict of interest.

Ethical approval

All experiments followed “Guiding Principles for the Care and Use of Animals in the Field of Physiological Sciences” and were approved by the animal care committee of Université du Québec à Trois-Rivières, in accordance with the Canadian Council on Animal Care.

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Copyright information

© The Physiological Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ChiropracticUniversité du Québec à Trois-RivièresTrois-RivièresCanada
  2. 2.CogNAC Research GroupUniversité du Québec à Trois-RivièresTrois-RivièresCanada
  3. 3.Department of AnatomyUniversité du Québec à Trois-RivièresTrois-RivièresCanada

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