Possible involvement of central oxytocin in cisplatin-induced anorexia in rats
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During cancer chemotherapy, drugs such as 5-HT3 receptor antagonists have typically been used to control vomiting and anorexia. We examined the effects of oxytocin (OXT), which has been linked to appetite, on cisplatin-induced anorexia in rats. Fos-like immunoreactivity (Fos-LI) expressed in the supraoptic nucleus (SON), the paraventricular nucleus (PVN), the area postrema and the nucleus of the solitary tract (NTS) after intraperitoneal (ip) administration of cisplatin. We also examined the fluorescence intensity of OXT-mRFP1 after ip administration of cisplatin in OXT-mRFP1 transgenic rats. The mRFP1 fluorescence intensity was significantly increased in the SON, the PVN, and the NTS after administration of cisplatin. The cisplatin-induced anorexia was abolished by OXT receptor antagonist (OXTR-A) pretreatment. In the OXT-LI cells, cisplatin-induced Fos expression in the SON and the PVN was also suppressed by OXTR-A pretreatment. These results suggested that central OXT may be involved in cisplatin-induced anorexia in rats.
KeywordsFos Hypothalamus Monomeric red fluorescent protein 1 (mRFP1) Paraventricular nucleus Supraoptic nucleus
We would like to thank Ms. Kanako Shoguchi and Ms. Yuki Nonaka (University of Occupational and Environmental Health, Kitakyushu, Japan) for their technical assistance. This study was supported by Grant-in-Aid for Scientific Research (C) number 16K08537 from the Japan Society for the Promotion of Science (JSPS), the Japan Agency for Medical Research and Development (AMED), and a research grant from Mitsubishi Tanabe Pharma Corporation.
KA: study design/experimental studies/figure preparation/manuscript preparation/statistical analysis; HH: study design/experimental studies/figure preparation/manuscript preparation/statistical analysis/manuscript review; SS: experimental studies/manuscript editing; HU: experimental studies/manuscript editing; RS: experimental studies/manuscript editing; YM: experimental studies/manuscript editing; MY: manuscript editing/experimental studies/manuscript review; TM: manuscript editing; KH: study design/guarantor of integrity of the study/manuscript editing; YU: study design/guarantor of integrity of the study; YU: study design/guarantor of integrity of the study/manuscript review/final approval.
Compliance with ethical standards
Conflict of interest
None of the authors has a conflict of interest to disclose.
This study was funded by Grant-in-Aid for Scientific Research (C) number 16K08537 from the Japan Society for the Promotion of Science (JSPS), the Japan Agency for Medical Research and Development (AMED), a and research grant from Mitsubishi Tanabe Pharma Corporation.
All procedures performed in this study involving animals were in accordance with the ethical standards of our institution or practice at which the studies were conducted under the control of the Ethics Committee of Animal Care and Experimentation, University of Occupational and Environmental Health, Japan.
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