Eicosapentaenoic acid triggers Ca2+ release and Ca2+ influx in mouse cerebral cortex endothelial bEND.3 cells
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Eicosapentaenoic acid (EPA), an omega-3 fatty acid abundant in fish oil, protects endothelial cells (EC) from lipotoxicity and triggers EC NO release. The latter is related to an elevation of cytosolic Ca2+. Although EPA has been shown to cause human EC cytosolic Ca2+ elevation, the mechanism is unclear. Microfluorimetric imaging was used here to measure free cytosolic Ca2+ concentration. EPA was shown to cause intracellular Ca2+ release in mouse cerebral cortex endothelial bEND.3 cells; interestingly, the EPA-sensitive intracellular Ca2+ pool(s) appeared to encompass and was larger than the Ca2+ pool mobilized by sarcoplasmic-endoplasmic reticulum Ca2+-ATPase inhibition by cyclopiazonic acid. EPA also opened a Ca2+ influx pathway pharmacologically distinct from store-operated Ca2+ influx. Surprisingly, EPA-triggered Ca2+ influx was Ni2+-insensitive; and EPA did not trigger Mn2+ influx. Further, EPA-triggered Ca2+ influx did not involve Na+–Ca2+ exchangers. Thus, our results suggest EPA triggered unusual mechanisms of Ca2+ release and Ca2+ influx in EC.
KeywordsEicosapentaenoic acid Endothelial cells Ca2+ release Ca2+ influx
Y.M.L, K.L.W and K.S.C would like to thank China Medical University, Taiwan, and the Ministry of Science and Technology of Taiwan for providing funding (103-2320-B-039-015-; 104-2320-B-039-030-; 104-2320-B-039-013-; 105-2320-B-039-028-; DMR-106-086; DMR-106-089). K.C.W would like to thank Chang Gung Memorial Hospital, Chiayi, for support (CMRPG6F0291).
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Conflict of interest
The authors declare no conflict of interests.
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