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The Journal of Physiological Sciences

, Volume 67, Issue 5, pp 613–622 | Cite as

Modulation of cutaneous extracellular collagen contraction by phosphorylation status of p130Cas

  • Mayumi Takeya
  • Yuushi Okumura
  • Takeshi NikawaEmail author
Original Paper

Abstract

Skin can respond to various types of internal and/or external mechanostimuli, such as excessive tension caused by body growth or decompression due to weight loss, which significantly affect skin morphology. Mechanosensors, including p130Cas, are reported to play a role in deformation and subsequent recovery of various tissues including skeletal muscles and blood vessels. However, the role of mechanotransduction via p130Cas in the regulation of skin size remains unclear. In this report, p130Cas activation was manipulated using a fibroblast-embedded collagen gel model or mouse skin contraction model. Inhibition or activation of Src family kinase-mediated phosphorylation of p130Cas significantly depressed and accelerated collagen gel contraction, respectively. The results also demonstrated age-dependent depression of cutaneous p130Cas activation in vivo. Inhibition of p130Cas signaling in our mouse model significantly suppressed recovery from cutaneous deformation. Taken together, our study highlighted the important role of p130Cas in cutaneous mechanotransduction for skin homeostasis.

Keywords

p130Cas Mechanotransduction Skin morphology Aging Extracellular matrix 

Abbreviations

CSK

C-terminal Srk kinase

ECM

Extracellular matrix

FAK

Focal adhesion kinase

FBS

Fetal bovine serum

HEK

Human embryonic kidney

MMP

Matrix metalloproteinase

p130Cas

p130 Crk-associated substrate

PP2

4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazolo [3,4-d] pyrimidine

Notes

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© The Physiological Society of Japan and Springer Japan 2016

Authors and Affiliations

  • Mayumi Takeya
    • 1
  • Yuushi Okumura
    • 1
    • 2
  • Takeshi Nikawa
    • 1
    Email author
  1. 1.Institute of Health BiosciencesThe University of Tokushima Graduate SchoolTokushimaJapan
  2. 2.Faculty of Nutritional ScienceSagami Women’s UniversityMinami-Ku SagamiharaJapan

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