The Journal of Physiological Sciences

, Volume 64, Issue 1, pp 1–11 | Cite as

GSK3β inhibition and LEF1 upregulation in skeletal muscle following a bout of downhill running

  • Hiral Amin
  • Judy Vachris
  • Alicia Hamilton
  • Nury Steuerwald
  • Reuben Howden
  • Susan Tsivitse ArthurEmail author
Original Paper


Canonical Wnt signaling is important in skeletal muscle repair but has not been well characterized in response to physiological stimuli. The objective of this study was to assess the effect of downhill running (DHR) on components of Wnt signaling. Young, male C57BL/J6 mice were exposed to DHR. Muscle injury and repair (MCadherin) were measured in soleus. Gene and protein expression of Wnt3a, active β-catenin, GSK3β, and LEF1 were measured in gastrocnemius. Muscle injury increased 6 days post-DHR and MCadherin protein increased 5 days post-DHR. Total and active GSK3β protein decreased 3 days (9-fold and 3.6-fold, respectively) post-DHR. LEF1 protein increased 6 days (5-fold) post-DHR. DHR decreased GSK3β and increased LEF1 protein expression, but did not affect other components of Wnt signaling. Due to their applicability, using models of physiological stimuli such as DHR will provide significant insight into cellular mechanisms within muscle.


DHR Skeletal muscle repair Wnt Exercise 



We thank Dr. S. Peter Magnusson for insightful feedback and discussion on the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© The Physiological Society of Japan and Springer Japan 2013

Authors and Affiliations

  • Hiral Amin
    • 2
  • Judy Vachris
    • 2
  • Alicia Hamilton
    • 2
  • Nury Steuerwald
    • 2
  • Reuben Howden
    • 1
  • Susan Tsivitse Arthur
    • 1
    Email author
  1. 1.Laboratory of Systems Physiology, Department of KinesiologyUNC CharlotteCharlotteUSA
  2. 2.Molecular Biology Core FacilityCannon Research CenterCharlotteUSA

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