The Journal of Physiological Sciences

, Volume 62, Issue 6, pp 453–460

The effects of gonadal steroid manipulation on the expression of Kiss1 mRNA in rat arcuate nucleus during postnatal development

  • Ken Takumi
  • Norio Iijima
  • Kinuyo Iwata
  • Shimpei Higo
  • Hitoshi Ozawa
Original Paper

Abstract

Kisspeptins, encoded by Kiss1 gene, play pivotal roles in the regulation of reproduction. Recently, several studies reported a sex difference in Kiss1 expression in the arcuate nucleus (ARC) during the neonatal period. In this study, we investigated the effect of gonadal steroid manipulation on the sex difference in Kiss1 expression in ARC of rats. At neonatal and prepubertal stages, females had a greater number of Kiss1 neurons than the males. Gonadectomy at those stages resulted in significant increases in the Kiss1 neuron number and the sex differences disappeared. We also confirmed the expression of estrogen receptor α in kisspeptin neurons in neonates. Altogether, our results indicate that ARC Kiss1 expression is negatively regulated by gonadal steroids from early postnatal stages, and that the sex difference in ARC Kiss1 expression is attributed to the difference in circulating gonadal steroid levels. We also found that neonatal estrogenization inhibits Kiss1 expression and impairs negative feedback system.

Keywords

Kisspeptin Arcuate nucleus Gonadal steroid Negative feedback Development 

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Copyright information

© The Physiological Society of Japan and Springer 2012

Authors and Affiliations

  • Ken Takumi
    • 1
  • Norio Iijima
    • 1
  • Kinuyo Iwata
    • 1
  • Shimpei Higo
    • 1
  • Hitoshi Ozawa
    • 1
  1. 1.Department of Anatomy and Neurobiology, Graduate School of MedicineNippon Medical SchoolTokyoJapan

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