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The Journal of Physiological Sciences

, Volume 60, Issue 5, pp 331–341 | Cite as

Phosphocreatine recovery overshoot after high intensity exercise in human skeletal muscle is associated with extensive muscle acidification and a significant decrease in phosphorylation potential

  • Jerzy A. ZoladzEmail author
  • Bernard Korzeniewski
  • Piotr Kulinowski
  • Justyna Zapart-Bukowska
  • Joanna Majerczak
  • Andrzej Jasiński
Original Paper

Abstract

The phosphocreatine (PCr) recovery overshoot in skeletal muscle is a transient increase of PCr concentration above the resting level after termination of exercise. In the present study [PCr], [ATP], [Pi] and pH were measured in calf muscle during rest, during plantar flexion exercise until exhaustion and recovery, using the 31P NMR spectroscopy. A significantly greater acidification of muscle cells and significantly lower phosphorylation potential (ΔG ATP) at the end of exercise was encountered in the group of subjects that evidenced the [PCr] overshoot as well as [ADP] and [Pi] undershoots than in the group that did not. We postulate that the role of the PCr overshoot-related transiently elevated [ATP]/[ADPfree] ratio is to activate different processes (including protein synthesis) that participate in repairing numerous damages of the muscle cells caused by intensive exercise-induced stressing factors, such as extensive muscle acidification, a significant decrease in ΔG ATP, an elevated level of reactive oxygen species or mechanical disturbances.

Keywords

31P NMR spectroscopy Acidosis Exercise Parallel activation PCr overshoot Skeletal muscle 

Notes

Acknowledgments

This study was supported by funding from the Polish Ministry of Science and Higher Education, grant no. N N404 196637. The authors thank Dr. Jacek Kibiński for his assistance during data collection.

Conflict of interest statement

There is no conflict of interest.

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Copyright information

© The Physiological Society of Japan and Springer 2010

Authors and Affiliations

  • Jerzy A. Zoladz
    • 1
    Email author
  • Bernard Korzeniewski
    • 2
  • Piotr Kulinowski
    • 3
  • Justyna Zapart-Bukowska
    • 1
  • Joanna Majerczak
    • 1
  • Andrzej Jasiński
    • 3
  1. 1.Department of Physiology and BiochemistryUniversity School of Physical Education-KrakówKrakowPoland
  2. 2.Faculty of Biochemistry, Biophysics and BiotechnologyJagiellonian UniversityKrakowPoland
  3. 3.Department of Nuclear RadiospectroscopyH. Niewodniczanski Institute of Nuclear PhysicsKrakowPoland

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