The effect of electroaucpuncture for 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced proteomic changes in the mouse striatum

  • Seung-Tae Kim
  • Woongjoon Moon
  • Younbyoung Chae
  • Youn Jung Kim
  • Hyejung Lee
  • Hi-Joon Park
Original Paper


Using a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) Parkinson’s disease mouse model, we investigated protein expression changes associated with the action of electroacupuncture (EA) in the mouse striatum. Twelve-week-old male C57BL/6 mice were injected intraperitoneally with 30 mg/kg of MPTP at 24-h intervals for 5 days, and the 100-Hz EA stimulation was performed at GB34 and GB39 once a day for 12 days consecutively from the first injection. With the EA, the MPTP-induced dopaminergic neuronal destruction was reduced. Of the 13 proteins that were differentially expressed between control and MPTP treated mice, cytosolic malate dehydrogenase, munc18-1, and hydroxyacylglutathione hydrolase, which were increased by MPTP, and cytochrome c oxidase subunit Vb, which was decreased by MPTP, were restored to the level of the saline group after EA treatment. These proteins are likely related to cellular metabolism. Altogether, we propose that the EA may exert neuroprotective effects in mice striatum through reducing MPTP-induced toxicity such as oxidative stress.


Acupuncture Electroacupuncture Hydroxyacylglutathione hydrolase 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Parkinson’s disease Striatum 



This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (R11-2005-014).


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Copyright information

© The Physiological Society of Japan and Springer 2009

Authors and Affiliations

  • Seung-Tae Kim
    • 1
  • Woongjoon Moon
    • 2
  • Younbyoung Chae
    • 2
  • Youn Jung Kim
    • 3
  • Hyejung Lee
    • 2
  • Hi-Joon Park
    • 2
  1. 1.Division of Meridian and Structural Medicine, School of Korean MedicinePusan National UniversityYangsan-siRepublic of Korea
  2. 2.Acupuncture and Meridian Science Research Center (AMSRC)Kyung Hee UniversitySeoulRepublic of Korea
  3. 3.East-West Nursing Research InstituteKyung Hee UniversitySeoulRepublic of Korea

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