Construction and application a vane system in a rotational rheometer for determination of the rheological properties of Monascus ruber CCT 3802
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The objectives of this work were the construction and adaptation of a vane system in a rotational rheometer; calibration of vane system using a mineral oil, and determination of rheological properties of the broth fermentation of Monascus ruber CCT 3802 at high cell density. Batch fermentation was carried out with glucose as the sole carbon source in a Bioflo III bioreactor. The consistency index (K) and flow behavior index (n) of the broth fermentation of M. ruber were characterized at different biomass concentrations and were adequately described by a power law model. The K and n values were significantly affected by biomass concentrations. K values ranged from 0.375 to 11.002 Pa sn when evaluated at biomass concentrations from 25.67 to 63.20 g L−1. The pseudoplastic behavior was confirmed by values of n that ranged between 0.157 and 0.254. Simple empirical correlations have been proposed to quantify the dependence of the power law terms on fungal biomass concentration.