Effect of advanced platelet-rich fibrin on accelerating alveolar bone formation in dogs: a histological and immunofluorescence evaluation

  • Masahiro To
  • Chen-yao Su
  • Kouki Hidaka
  • Toshimitsu Okudera
  • Masato MatsuoEmail author
Original Article


Several methods have been developed to regenerate lost alveolar bone. Platelet-rich fibrin (PRF) is a useful adjunct for new bone formation in dentistry. To elucidate the effect of advanced PRF (A-PRF) on bone formation, we inserted A-PRF clots in sockets after tooth extraction. Premolars were extracted from beagle dogs, and A-PRF was applied to the socket. New bone formation was assessed using histological and immunofluorescence examinations, and the bone formation ratio was evaluated 14 and 30 days postoperatively. Histological examination revealed newly formed bone filling the sockets up to the center in the A-PRF group at 14 days postoperatively, while thick and regular bone trabeculae were arranged in porous bone after 30 days. Higher expressions of osteocalcin and osteopontin were observed in newly formed bone in the A-PRF group, compared to the control group. The bone formation ratio was also higher in the A-PRF group than in the control group. Thus, A-PRF application may result in enhanced new bone formation and may aid in accelerating bone formation. A-PRF was more rapid than a self-limiting process during induction of bone formation by enhancing osteoblast activity and may be useful for bone formation in clinical medicine.


Alveolar bone Bone formation Dog Osteocalcin Osteopontin Platelet-rich fibrin 



This research was partially supported by the Japan Society for the Promotion of Science and a Grant-in-Aid for Scientific Research (C) under Grant number 17K11632 to M. M.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Japanese Association of Anatomists 2019

Authors and Affiliations

  • Masahiro To
    • 1
  • Chen-yao Su
    • 1
    • 2
  • Kouki Hidaka
    • 1
  • Toshimitsu Okudera
    • 1
  • Masato Matsuo
    • 1
    Email author
  1. 1.Department of Oral ScienceGraduate School of Dentistry, Kanagawa Dental UniversityYokosukaJapan
  2. 2.Department of DentistryNational Yang Ming UniversityTaipeiTaiwan

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