Anatomical Science International

, Volume 92, Issue 4, pp 554–568 | Cite as

Anatomy, histology and elemental profile of long bones and ribs of the Asian elephant (Elephas maximus)

  • Korakot Nganvongpanit
  • Puntita Siengdee
  • Kittisak Buddhachat
  • Janine L. Brown
  • Sarisa Klinhom
  • Tanita Pitakarnnop
  • Taweepoke Angkawanish
  • Chatchote Thitaram
Original Article

Abstract

This study evaluated the morphology and elemental composition of Asian elephant (Elephas maximus) bones (humerus, radius, ulna, femur, tibia, fibula and rib). Computerized tomography was used to image the intraosseous structure, compact bones were processed using histological techniques, and elemental profiling of compact bone was conducted using X-ray fluorescence. There was no clear evidence of an open marrow cavity in any of the bones; rather, dense trabecular bone was found in the bone interior. Compact bone contained double osteons in the radius, tibia and fibula. The osteon structure was comparatively large and similar in all bones, although the lacuna area was greater (P < 0.05) in the femur and ulna. Another finding was that nutrient foramina were clearly present in the humerus, ulna, femur, tibia and rib. Twenty elements were identified in elephant compact bone. Of these, ten differed significantly across the seven bones: Ca, Ti, V, Mn, Fe, Zr, Ag, Cd, Sn and Sb. Of particular interest was the finding of a significantly larger proportion of Fe in the humerus, radius, fibula and ribs, all bones without an open medullary cavity, which is traditionally associated with bone marrow for blood cell production. In conclusion, elephant bones present special characteristics, some of which may be important to hematopoiesis and bone strength for supporting a heavy body weight.

Keywords

Bone CT scan Elephant Mineral Osteon 

Abbreviations

Mg

Magnesium (12)

Al

Aluminum (13)

Si

Silicon (14)

P

Phosphorus (15)

S

Sulfur (16)

K

Potassium (19)

Ca

Calcium (20)

Ti

Titanium (22)

V

Vanadium (23)

Cr

Chromium (24)

Mn

Manganese (25)

Fe

Iron (26)

Ni

Nickel (28)

Cu

Copper (29)

Zn

Zinc (30)

Zr

Zirconium (30)

Ag

Silver (47)

Cd

Cadmium (48)

Sn

Tin (50)

Sb

Antimony (51)

pb

Lead (82)

LE

Light element from H = hydrogen (1) to Na = sodium (11)

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Copyright information

© Japanese Association of Anatomists 2016

Authors and Affiliations

  • Korakot Nganvongpanit
    • 1
    • 2
  • Puntita Siengdee
    • 1
  • Kittisak Buddhachat
    • 1
  • Janine L. Brown
    • 3
  • Sarisa Klinhom
    • 2
  • Tanita Pitakarnnop
    • 1
  • Taweepoke Angkawanish
    • 4
  • Chatchote Thitaram
    • 2
  1. 1.Animal Bone and Joint Research Laboratory, Department of Veterinary Biosciences and Public Health, Faculty of Veterinary MedicineChiang Mai UniversityChiang MaiThailand
  2. 2.Faculty of Veterinary Medicine, Center of Excellence in Elephant Research and EducationChiang Mai UniversityChiang MaiThailand
  3. 3.Smithsonian Conservation Biology Institute, Center for Species SurvivalFront RoyalUSA
  4. 4.National Elephant Institute, Forest Industry OrganizationLampangThailand

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