Anatomical Science International

, Volume 90, Issue 1, pp 47–53 | Cite as

Effect of Boswellia serrata gum resin on the morphology of hippocampal CA1 pyramidal cells in aged rat

  • Mohammad Hosseini-sharifabadEmail author
  • Ebrahim Esfandiari
Original Article


Experimental evidence indicates that administration of Boswellia resin, known as olibanum or Frankincense, increases memory power. It is reported that beta boswellic acid, the major component of Boswellia serrata gum resin, could enhance neurite outgrowth and branching in hippocampal neurons. We therefore studied whether Boswellia treatment produces morphological changes in the superior region of cornu ammonis (CA1) in aged rats. Sixteen male Wistar rats, 24 months of age, were randomly divided in experimental and control groups. The experimental group was orally administered Boswellia serrata gum resin (100 mg/kg per day for 8 weeks) and the control group received a similar volume of water. The Cavalieri principle was employed to estimate the volumes of CA1 hippocampal field, and a quantitative Golgi study was used to analysis of dendritic arborizations of CA1 pyramidal cells. Comparisons revealed that Boswellia-treated aged rats had greater volumes than control animals in stratum pyramidale and stratum radiatum lacunosum-moleculare. The neurons of CA1 in experimental rats had more dendritic segments (40.25 ± 4.20) than controls (30.9 ± 4.55), P = 0.001. The total dendritic length of CA1 neurons was approximately 20 % larger in the experimental group compared to control. Results also indicated that the aged rats treated with Boswellia resin had more numerical branching density in the apical dendrites of CA1 pyramidal neurons. The results of the present study show that long-term administration of Boswellia resin can attenuate age-related dendritic regression in CA1 pyramidal cells in rat hippocampus.


Aging Boswellia Dendrite Hippocampus Rat 



This study was funded by the research deputy of Shahid Sadoughi University of Medical Sciences, (Research project No. 1520). The authors gratefully acknowledge the Department of Biomedical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran, for providing excellent working facilities.

Conflict of interest



  1. Ammon HP (2006) Boswellic acids in chronic inflammatory diseases. Planta Med 12:1100–1116CrossRefGoogle Scholar
  2. Archier P, Vieillescazes C (2000) Characterization of various geographical origin incense based on chemical criteria. Analysis 28:233–237CrossRefGoogle Scholar
  3. Camarda L, Dayton T, Di Stefano V, Pitonzo R, Schillaci D (2007) Chemical composition and antimicrobial activity of some oleogum resin essential oils from Boswellia spp. (Burseraceae). Ann Chim 97:837–844PubMedCrossRefGoogle Scholar
  4. De Brabander JM, Kramers RJ, Uylings HB (1998) Layer-specific dendritic regression of pyramidal cells with ageing in the human prefrontal cortex. Eur J Neurosci 10:1261–1269PubMedCrossRefGoogle Scholar
  5. De Ruiter JP, Uylings HBM (1987) Morphometric and dendritic analysis of fascia dentata granule cells in human aging and senile dementia. Brain Res 402:217–229PubMedCrossRefGoogle Scholar
  6. Dixit VP, Joshi S, Sinha R, Bharva VS, Varma M (1980) Hypolipidemic activity of guggal resin and garlic in dogs and monkeys. Biochem Exp Biol 16:421–424PubMedGoogle Scholar
  7. Driscoll I, Sutherland RJ (2005) The aging hippocampus: navigating between rat and human experiments. Rev Neurosci 16:87–121PubMedCrossRefGoogle Scholar
  8. Duwiejua M, Zeitlin IJ, Waterman PG, Chapman J (1993) Anti-inflammatory activity of resins from some species of the plant family Burseracea. Planta Med 59:6–12CrossRefGoogle Scholar
  9. Ernst E (2008) Frankincense: systematic review. BMJ 17(337):a2813CrossRefGoogle Scholar
  10. Farshchi A, Ghiasi G, Farshchi S, Malek Khatabi P (2010) Effects of Boswellia Papyrifera gum extract on learning and memory in mice and rats. Iran J Basic Med Sci 13:9–15Google Scholar
  11. Gabbott PL, Somogyi J (1994) The single section Golgi impregnation procedure: methodological description. J Neurosci Methods 11:221–230CrossRefGoogle Scholar
  12. Gundersen HJG, Bendtsen TF, Korbo L et al (1988) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 96:379–394PubMedCrossRefGoogle Scholar
  13. Himeda T, Mizuno K, Kato H, Araki T (2005) Effects of age on immunohistochemical changes in the mouse hippocampus. Mech Ageing Dev 126:673–677PubMedCrossRefGoogle Scholar
  14. Horner CH (1993) Plasticity of the dendritic spine. Prog Neurobiol 41:281–321PubMedCrossRefGoogle Scholar
  15. Hosseini M, Hadjzad M, Derekhshan M et al (2010) The beneficial effects of olibanum on memory deficit induced by hypothyroidism in adult rats tested in Morris water maze. Arch Pharm Res 33:463–468PubMedCrossRefGoogle Scholar
  16. Hsieh MT, Peng WH, Wu CR, Ng KY, Cheng CL, Xu HX (2010) Review on experimental research of herbal medicines with anti-amnesic activity. Planta Med 76:203–217PubMedCrossRefGoogle Scholar
  17. Karima O, Riazi G, Yousefi R, Moosavi Movahedi AA (2010) The enhancement effect of beta-boswellic acid on hippocampal neurites outgrowth and branching (an in vitro study). Neurol Sci 31:315–320PubMedCrossRefGoogle Scholar
  18. Karima O, Riazi G, Khodadadi S et al (2012) An in vitro study of the role of β-boswellic acid in the microtubule assembly dynamics. FEBS Lett 586:4132–4138PubMedCrossRefGoogle Scholar
  19. Mahmoudi A, Hosseini-Sharifabad A, Monsef-Esfahani HR et al (2011) Evaluation of systemic administration of Boswellia papyrifera extracts on spatial memory retention in male rats. J Nat Med 65:519–525PubMedCrossRefGoogle Scholar
  20. Markham JA, McKian KP, Stroup TS, Juraska JM (2005) Sexually dimorphic aging of dendritic morphology in CA1 of hippocampus. Hippocampus 15:97–103PubMedCrossRefGoogle Scholar
  21. Menon MK, Kar A (1971) Analgesic and psychopharmacological effects of the gum resin of Boswellia serrata. Planta Med 19:333–341PubMedCrossRefGoogle Scholar
  22. Moussaieff A, Mechoulam R (2009) Boswellia resin: from religious ceremonies to medical uses; a review of in vitro, in vivo and clinical trials. J Pharm Pharmacol 61:1281–1293PubMedCrossRefGoogle Scholar
  23. Moussaieff A, Shohami E, Kashman Y et al (2007) Incensole acetate, a novel anti-inflammatory compound isolated from Boswellia resin, inhibits nuclear factor-kappaB activation. Mol Pharmacol 72:1657–1664PubMedCrossRefGoogle Scholar
  24. Moussaieff A, Shein NA, Tsenter J et al (2008a) Incensole acetate-a novel neuroprotective agent isolated from Boswellia carterii. J Cereb Blood Flow Metab 28:1341–1352PubMedCrossRefGoogle Scholar
  25. Moussaieff A, Rimmerman N, Bregman T et al (2008b) Incensole acetate, an incense component, elicits psychoactivity by activating TRPV3 channels in the brain. FASEB J 22:3024–3034PubMedCentralPubMedCrossRefGoogle Scholar
  26. Mt Huan, Badmaev V, Ding Y, Liu Y, Xie JG, Ho CT (2000) Anti-tumor and anti carcinogenic activities of triterpenoid, beta boswellic acid. BioFactors 13:225–230CrossRefGoogle Scholar
  27. Nakayama T, Sawada T (2002) Involvement of microtubule integrity in memory impairment caused by colchicines. Pharmacol Biochem Behav 71:119–138PubMedCrossRefGoogle Scholar
  28. Paxinos G, Watson C (2007) The rat brain in stereotaxic coordinates, 6th edn. Academic Press, New YorkGoogle Scholar
  29. Poeckel D, Werz O (2002) Boswellic acids: biological actions and molecular targets. Curr Med Chem 13:3359–3369CrossRefGoogle Scholar
  30. Rosenzweig ES, Barnes CA (2003) Impact of aging on hippocampal function: plasticity, network dynamics, and cognition. Prog Neurobiol 69:143–179PubMedCrossRefGoogle Scholar
  31. Sharma ML, Bani S, Singh GB (1989) Anti-arthritic activity of boswellic acids in bovine serum albumin (BSA)-induced arthritis. Int J Immunopharmacol 11:647–652PubMedCrossRefGoogle Scholar
  32. Uemura E, Carriquiry A, Kliemann W, Goodwin J (1995) Mathematical modelling of dendritic growth in vitro. Brain Res 671:187–194PubMedCrossRefGoogle Scholar
  33. Uylings HB, De Brabander JM (2002) Neuronal changes in normal human aging and Alzheimer’s disease. Brain Cogn 49:268–276PubMedCrossRefGoogle Scholar
  34. Uylings HB, Van Pelt J (2002) Measures for quantifying dendritic arborizations. Network 13:397–414PubMedCrossRefGoogle Scholar
  35. Uylings HB, Ruiz-Marcos A, Van Pelt J (1986) The metric analysis of three-dimensional dendritic patterns: a methodological review. J Neurosci Methods 18:127–151PubMedCrossRefGoogle Scholar
  36. West MJ, Slomianka L, Gundersen HJG (1991) Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator. Anat Rec 231:482–497PubMedCrossRefGoogle Scholar

Copyright information

© Japanese Association of Anatomists 2014

Authors and Affiliations

  • Mohammad Hosseini-sharifabad
    • 1
    Email author
  • Ebrahim Esfandiari
    • 2
  1. 1.Department of Biology and Anatomical SciencesShahid Sadoughi University of Medical SciencesYazdIran
  2. 2.Department of Anatomical Sciences and Molecular BiologyIsfahan University of Medical SciencesIsfahanIran

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