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Dynamic distribution of intestinal microbes in Schizothorax o’connori at different growth stages

  • Zhenda ShangEmail author
  • Qinghui Kong
  • Suozhu Liu
  • Zhankun Tan
  • Peng Shang
  • Honghui Wang
Original Article Aquaculture

Abstract

Gut microbes are closely related to physical health. The intestinal microbial population in fish is a complicated ecological environment that plays an important role in the digestion and absorption of nutrients and fish health. As a unique species in Tibet, Schizothorax o’connori has a protective value for the ecological balance of plateau rivers. However, with the reproduction of invasive fish species, the population of S. o’connori has declined significantly. Therefore, we used 16S ribosomal DNA (rDNA) sequencing techniques to analyze the composition and diversity of intestinal microbiota in S. o’connori at different growth stages, which can contribute to sustainable reproduction and survival of S. o’connori. The results showed that the diversity of intestinal microflora increased with the growth of S. o’connori. A total of 24 phyla, including the four predominant phyla Proteobacteria, Verrucomicrobia, Firmicutes, and Bacteroidetes, were detected, and 292 genera, including the three predominant genera Pseudomonas, Moraxellaceae, and Geobacillus, were identified. These results confirm that the ability to decompose fibers and utilize carbohydrates in feeds was enhanced. Intestinal health and immunization status were improved with the growth of S. o’connori. Different growth stages significantly affected the microbial community structure of the intestinal tract of S. o’connori.

Keywords

Schizothorax o’connori Different growth stages Intestinal microbial 16S rDNA sequencing techniques PICRUSt functional 

Notes

Acknowledgements

This research was supported by the Tibetan Plateau Feed Processing Research Center Project (XZJYT2018GCZX), Natural Science Foundation of China (31760673), and Central Government Supports Local Colleges and Universities to Develop Special Funds Projects (ZZXT2019-02). The organizational unit was the Tibet Agricultural and Animal Husbandry University.

Compliance with ethical standards

Conflict of interest

We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

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Copyright information

© Japanese Society of Fisheries Science 2019

Authors and Affiliations

  • Zhenda Shang
    • 1
    • 2
    Email author
  • Qinghui Kong
    • 1
    • 2
  • Suozhu Liu
    • 1
    • 2
  • Zhankun Tan
    • 1
    • 2
  • Peng Shang
    • 1
    • 3
  • Honghui Wang
    • 1
    • 2
  1. 1.College of Animal ScienceTibet Agricultural and Animal Husbandry UniversityNyingchiChina
  2. 2.Tibetan Plateau Feed Processing Research CenterTibet Agricultural and Animal Husbandry UniversityNyingchiChina
  3. 3.Tibetan pig Collaborative Research Center (Open platform)NyingchiChina

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