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Ingestion, digestion, growth and survival of green mussel Perna viridis pediveliger larvae fed different microalgae

Abstract

Nutrition plays a critical role in the growth and development of green mussel Perna viridis larvae. In this study, three species of tropical microalgae were investigated to determine their efficacy as feed source for the pediveliger stage. Larvae (2 larvae ml−1) were given Isochrysis galbana (Iso), Chaetoceros calcitrans (Cc), and Tetraselmis tetrahele (Tt) at 100,000 cells ml−1. No statistically significant difference in survival was found among treatments. The Cc diet promoted maximum growth in terms of final shell height and shell length, as well as instantaneous growth rate. Filtration rates were high, but were not significantly different. A significantly higher ingestion rate was observed in Cc-fed compared with Iso- and Tt-fed larvae. Moreover, all diets examined were readily ingested and digested by the larvae, with faster cell digestion in Cc-fed larvae based on epifluorescence microscopy. This study demonstrated that C. calcitrans, I. galbana, and T. tetrahele were all acceptable and digestible for the animals, but in terms of growth parameters and filtration and ingestion rates, C. calcitrans performed best. Information on food digestibility and feeding habits of green mussel will provide us with a better understanding of the appropriate food to be given that will suit their feeding biology and improve their production in captivity.

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Acknowledgements

The authors would like to thank the Department of Science and Technology‒Science and Education Institute‒Accelerated Science and Technology Human Resource Development Program (DOST-SEI-ASTHRDP), Philippine Council for Agriculture, Aquatic and Natural Resources, Research and Development‒Graduate Research and Education Assistantship for Technology Program (DOST-PCAARRD-GREAT) and University of the Philippines Visayas Office of the Vice Chancellor for Research and Extension (UPV-OVCRE) for the grants. Gratitude is also extended to Southeast Asian Fisheries Development Center, Aquaculture Department (SEAFDEC/AQD) for the use of facilities, and the technical support of Mr. Argie Nicmic and Mr. Joel Fabrigas in the culture of natural food, Engr. Margarita Arnaiz and Mr. Norwell Bautista in using the epifluorescence microscope, Ms. Shiela Untalan and Mr. Jessie Jimenez for their assistance in the conduct of the study, Dr. Liberato Laureta for the comments and suggestions to improve this manuscript and Mr. Roman Sanares for the statistical analysis.

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Correspondence to Fiona L. Pedroso.

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Maquirang, J.R.H., Pedroso, F.L., Apines-Amar, M.J. et al. Ingestion, digestion, growth and survival of green mussel Perna viridis pediveliger larvae fed different microalgae. Fish Sci 86, 97–105 (2020). https://doi.org/10.1007/s12562-019-01367-3

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Keywords

  • Bivalve
  • Chaetoceros calcitrans
  • Filtration rate
  • Epifluorescence microscopy
  • Ingestion rate
  • Isochrysis galbana
  • Tetraselmis tetrahele