Fisheries Science

, Volume 86, Issue 1, pp 107–118 | Cite as

Effects of complete replacement of fish oil with plant oil mixtures and algal meal on growth performance and fatty acid composition in juvenile yellowtail Seriola quinqueradiata

  • Haruhisa FukadaEmail author
  • Renato Kitagima
  • Junpei Shinagawa
  • Haruka Morino
  • Toshiro Masumoto
Original Article Aquaculture


Docosahexaenoic acid (DHA) is an essential fatty acid for marine carnivorous fish. Algal meal (AM), available as a new dietary DHA source, could completely replace dietary fish oil (FO). In this study, dietary FO was replaced with plant oil mixtures and AM in juvenile yellowtail Seriola quinqueradiata to investigate its effects on growth performance and fatty acid composition. The FO control diet was prepared with only pollack liver oil as the lipid source. For the non-FO diets, pollack liver oil was completely replaced with mixtures of canola oil and palm oil, with AM supplementation at 0% (AM0), 1% (AM1), 2% (AM2), 3% (AM3), and 4% (AM4). After completion of the 8-week feeding trial, the AM2 group showed significantly higher values for final body weight and feed efficiency than the AM0 group. No significant differences were observed in the other parameters of growth performance. Whole-body fatty acid composition reflected the dietary fatty acid composition in all dietary groups. These findings demonstrate that AM is useful as a DHA source in yellowtail aquaculture, thus contributing to a reduction in the use of FO in fish diets.


Algal meal Fatty acid Fish oil replacement Yellowtail 



This study was funded by Alltech (USA and Japan) through a joint research alliance between Alltech and Kochi University, Japan.


  1. Aoki H, Sanada Y, Furuishi M, Kimoto R, Maita M, Akimoto A, Yamagata Y, Watanabe T (2000) Partial or complete replacement of fish meal by alternate protein sources in diets for yellowtail and red sea bream. Aquacult Sci 48:53–63Google Scholar
  2. Bowyer JN, Qin JG, Smullen RP, Srone DAJ (2012) Replacement of fish oil by poultry oil and canola oil in yellowtail kingfish (Seriola lalandi) at optimal and suboptimal temperatures. Aquaculture 356–357:211–222CrossRefGoogle Scholar
  3. Brignol FD, Fernandes VAG, Nobrega RO, Corrêa CF, Filler K, Pettigrew J, Fracalossi DM (2018) Aurantiochytrium sp. meal as DHA source in Nile tilapia diet, part II: Body fatty acid retention and muscle fatty acid profile. Aquacult Res 50:707–716Google Scholar
  4. Byreddy AR (2016) Thraustochytrids as an alternative source of omega-3 fatty acids, carotenoids and enzymes. Lipid Tech 28:68–70CrossRefGoogle Scholar
  5. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306CrossRefGoogle Scholar
  6. Deshimaru O, Kuroki K, Yone Y (1982) Suitable levels of lipids and ursodesoxycholic acid in diet for yellowtail. Nippon Suisan Gakkaishi 48:1265–1270CrossRefGoogle Scholar
  7. Fernandes VAG, Brignol FD, Filler K, Pettigrew J, Fracalossi DM (2018) Aurantiochytrium sp. meal as DHA source in Nile tilapia diet, part I: Growth performance and body composition. Aquacult Res 50:390–399CrossRefGoogle Scholar
  8. Folch J, Lees M, Stanley SGH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226:497–509Google Scholar
  9. Frøyland L, Vaagenes H, Asiedu DK, Garras A, Lie Ø, Berge RK (1996) Chronic administration of eicosapentaenoic acid and docosahexaenoic acid as ethyl esters reduced plasma cholesterol and changed the fatty acid composition in rat blood and organs. Lipids 31:169–178CrossRefGoogle Scholar
  10. Frøyland L, Madsen L, Vaagenes H, Totland GK, Auwerx J, Kryvi H, Staels B, Berge RK (1997) Mitochondrion is the principal target for nutritional and pharmacological control of triglyceride metabolism. J Lipid Res 38:1851–1858PubMedGoogle Scholar
  11. Fukada H, Taniguchi E, Morioka K, Masumoto T (2017) Effects of replacing fish oil with canola oil on the growth performance, fatty acid composition and metabolic enzyme activity of juvenile yellowtail Seriola quinqueradiata (Temminck & Schlegel, 1845). Aquacult Res 48:5928–5939CrossRefGoogle Scholar
  12. García-Ortega A, Kissinger KR, Trushenski JT (2016) Evaluation of fish meal and fish oil replacement by soybean protein and algal meal from Schizochytrium limacinum in diets for giant grouper Epinephelus lanceolatus. Aquaculture 452:1–8CrossRefGoogle Scholar
  13. Hardy RW (2010) Utilization of plant proteins in fish diets: effects of global demand and supplies of fishmeal. Aquacult Res 41:770–776CrossRefGoogle Scholar
  14. Henderson RJ, Sargent JR (1985) In Nutrition and Feeding in Fish (CB Cowey, AM Mackie and JG Bell, Eds) Fatty acid metabolism in fish: 349– 364Google Scholar
  15. Henderson RJ, Tocher DR (1987) The lipid composition and biochemistry of freshwater fish. Prog Lipid Res 26:281–347CrossRefGoogle Scholar
  16. Kissinger KR, García-Ortega A, Trushenski JT (2016) Partial fish meal replacement by soy protein concentrate, squid and algal meals in low fish-oil diets containing Schizochytrium limacinum for longfin yellowtail Seriola rivoliana. Aquaculture 452:37–44CrossRefGoogle Scholar
  17. Kousoulaki K, Østbye T-KK, Krasnov A, Torgersen JS, Mørkøre T, Sweetman J (2015) Metabolism, health and fillet nutritional quality in Atlantic salmon (Salmo salar) fed diets containing n-3-rich microalgae. J Nutr Sci 4:e24CrossRefGoogle Scholar
  18. Lee MC, Zhuo LC, Lin Y-H (2017) Effects of dietary docosahexaenoic acid sources, microalgae meal and oil, on growth, fatty acid composition and docosahexaenoic acid retention of orange-spotted grouper, Epinephelus coioides. Aquacult Res 49:30–35CrossRefGoogle Scholar
  19. Masumoto T (2002) Yellowtail, Seriola quinqueradiata (CD Webster and CE Lim, Eds.). Nutrient requirements and feeding of finfish for Aquaculture:131–146Google Scholar
  20. Masumoto T, Ruchimat T, Ito Y, Hosokawa H, Shimeno S (1996) Amino acid availability values for several protein sources for yellowtail (Seriola quinqueradiata). Aquaculture 146:109–119CrossRefGoogle Scholar
  21. Miller MR, Nichols PD, Carter CG (2007) Replacement of fish oil with thraustochytrid Schizochytrium sp. L oil in Atlantic salmon parr (Salmo salar L) diets. Comp Biochem Physiol A 148:382–392CrossRefGoogle Scholar
  22. Park W, Moon M, Shina S, Cho JM, Suh WI, Chang YK, Lee B (2018) Economical DHA (Docosahexaenoic acid) production from Aurantiochytrium sp. KRS101 using orange peel extract and low cost nitrogen sources. Algal Res 29:71–79CrossRefGoogle Scholar
  23. Qiao H, Wang H, Song Z, Ma J, Li B, Liu X, Zhang S, Wang J, Zhang L (2014) Effects of dietary fish oil replacement by microalgae raw materials on growth performance, body composition and fatty acid profile of juvenile olive flounder, Paralichthys olivaceus. Aquacult Nutr 20:646–653CrossRefGoogle Scholar
  24. Qu L, Ren LJ, Huang H (2013) Scale-up of docosahexaenoic acid production in fed-batch fermentation by Schizochytrium sp. based on volumetric oxygen-transfer coefficient. Biochem Eng J 77:82–87CrossRefGoogle Scholar
  25. Ratledge C (2004) Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie 86:807–815CrossRefGoogle Scholar
  26. Ryu BG, Kim K, Kim J, Han JI, Yang JW (2013) Use of organic waste from the brewery industry for high-density cultivation of the docosahexaenoic acid-rich microalga, Aurantiochytrium sp. KRS101. Biores Technol 129:351–359CrossRefGoogle Scholar
  27. Sajjadi B, Chen WY, Raman AAA, Ibrahim S (2018) Microalgae lipid and biomass for biofuel production: A comprehensive review on lipid enhancement strategies and their effects on fatty acid composition. Renew Sustain Energy Rev 97:200–232CrossRefGoogle Scholar
  28. Sargent JR, Tocher DR, Bell JG (2002) The lipids. In: Halver JE, Hardy RW (eds) Fish nutrition, 3rd edn. New York, pp 181–257Google Scholar
  29. Sarker PK, Gamble MM, Kelson S, Kapuscinski AR (2015) Nile tilapia (Oreochromis niloticus) show high digestibility of lipid and fatty acids from marine Schizochytrium sp. and of protein and essential amino acids from freshwater Spirulina sp. feed ingredients. Aquacult Nutr 22:109–119CrossRefGoogle Scholar
  30. Sarker PK, Kapuscinski AR, Lanois AJ, Livesey ED, Bernhard KP, Coley ML (2016) Towards sustainable aquafeeds: complete substitution of fish oil with marine microalga Schizochytrium sp. improves growth and fatty acid deposition in juvenile Nile tilapia (Oreochromis niloticus). (JL Soengas, Ed.). PLoS One 11:e0156684Google Scholar
  31. Seno-o A, Takakuwa F, Hashiguchi T, Morioka K, Masumoto T, Fukada H (2008) Replacement of dietary fish oil with olive oil in young yellowtail Seriola quinqueradiata: effects on growth, muscular fatty acid composition and prevention of dark muscle discoloration during refrigerated storage. Fish Sci 74:1297–1306CrossRefGoogle Scholar
  32. Shinagawa J, Morino H, Masumoto T, Fukada H (2017) Development of a docosahexaenoic acid (DHA)-rich yellowtail Seriola quinqueradiata using tuna by-product oil. Fish Sci 83:607–617CrossRefGoogle Scholar
  33. Song X, Zhang X, Zhang X (2015) Production of High Docosahexaenoic acid by Schizochytrium sp. using low-cost raw materials from food industry. J Oleo Sci 64:197–204CrossRefGoogle Scholar
  34. Sprague M, Walton J, Campbell PJ, Strachan F, Dick JR, Bell JG (2015) Replacement of fish oil with a DHA-rich algal meal derived from Schizochytrium sp. on the fatty acid and persistent organic pollutant levels in diets and flesh of Atlantic salmon (Salmo salar, L.) post-smolts. Food Chem 185:413–421CrossRefGoogle Scholar
  35. Takeda M, Shimeno S, Hosokawa H, Amano K, Ikeda K, Inoue I (1989) Effects of supplemental dietary oxidized oil and nutrient mixture on lipid peroxidation in red sea bream. Suisanzousyoku 37:1–7Google Scholar
  36. Takeuchi T, Toyota M, Satoh S, Watanabe T (1990) Requirement of juvenile red seabream Pagrus major for eicosapentaenoic and docosahexaenoic acids. Nippon Suisan Gakkaishi 56:1263–1269CrossRefGoogle Scholar
  37. Tocher DR (2010) Fatty acid requirements in ontogeny of marine and freshwater fish. Aquacult Res 41:717–732CrossRefGoogle Scholar
  38. Tonial IB, Stevanato FB, Matsushita M, De Souza NE, Furuya WM, Visentainer JV (2009) Optimization of flaxseed oil feeding time length in adult Nile tilapia (Oreochromis niloticus) as a function of muscle omega-3 fatty acids composition. Aquacult Nutr 15:564–568CrossRefGoogle Scholar
  39. Totland GK, Madsen L, Klemetsen B, Vaagenes H, Kryvi H, Frøyland L, Hexeberg S, Berge RK (2000) Proliferation of mitochondria and gene expression of carnitine palmitoyl transferase and fatty acyl-CoA oxidase in rat skeletal muscle, heart and liver by hypolipidemic fatty acids. Biol Cell 92:1–13CrossRefGoogle Scholar
  40. Turchini GM, Torstensen BE, Ng WK (2009) Fish oil replacement in finfish nutrition. Rev Aquacult 1:10–57CrossRefGoogle Scholar
  41. Watanabe T (2002) Strategies for further development of aquatic feeds. Strategies for further development of aquatic feeds. Fish Sci 68:242–252CrossRefGoogle Scholar
  42. Xu H, Cao L, Wei Y, Zhang Y, Liang M (2018) Lipid contents in farmed fish are influenced by dietary DHA/EPA ratio: a study with the marine flatfish, tongue sole (Cynoglossus semilaevis). Aquaculture 485:183–190CrossRefGoogle Scholar

Copyright information

© Japanese Society of Fisheries Science 2019

Authors and Affiliations

  1. 1.Faculty of Agriculture and Marine ScienceKochi UniversityNankokuJapan
  2. 2.Alltech JapanFukuokaJapan

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