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Developmental expression of LPXRFa, kisspeptin, and their receptor mRNAs in the half-smooth tongue sole Cynoglossus semilaevis

  • Bin Wang
  • Yaxing Zhang
  • Yongjiang Xu
  • Xuezhou LiuEmail author
  • Aijun Cui
  • Bao Shi
  • Yan Jiang
Original Article Biology

Abstract

Both gonadotropin-inhibitory hormone (GnIH) and kisspeptin play a critical role in the control of reproduction in vertebrates. However, the spatial–temporal expression patterns and possible roles of these two peptides during development are poorly understood. To this end, we evaluated dynamic expression profiles of lpxrfa, the piscine ortholog of gnih, kisspeptin (kiss2) and their receptor mRNAs during embryogenesis and early larval development in a flatfish, Cynoglossus semilaevis. Quantitative RT-PCR analysis indicated that both ligand (lpxrfa and kiss2) and receptor (lpxrfa-r and kiss2r) mRNAs were detected in unfertilized eggs and during embryogenesis, but with different expression profiles. In addition, lpxrfa-r, kiss2 and kiss2r transcripts increased significantly at 6 days post-hatching (dph), while lpxrfa mRNAs did not vary significantly during all larval development. Taken together, these results of the present study suggest that both LPXRFa and kisspeptin systems could be functional and play differential roles during embryogenesis and early larval development in tongue sole.

Keywords

LPXRFa Kisspeptin Early development Cynoglossus semilaevis 

Notes

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (31602133), the Natural Science Foundation of Shandong Province (ZR2016CB02), Qingdao Municipal Science and Technology Bureau (17-1-1-61-jch), the Central Public-interest Scientific Institution Basal Research Fund, CAFS (2017HY-XKQ01), and the China Agriculture Research System (CARS-47).

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Copyright information

© Japanese Society of Fisheries Science 2019

Authors and Affiliations

  1. 1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoChina
  2. 2.Laboratory for Marine Fisheries and Food Production ProcessesPilot National Laboratory for Marine Science and Technology (Qingdao)QingdaoChina
  3. 3.College of Fisheries and Life ScienceShanghai Ocean UniversityShanghaiChina

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