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Fisheries Science

, Volume 85, Issue 1, pp 157–165 | Cite as

Effects of dietary konjac oligosaccharide supplementation on serum immune parameters and intestinal immunity of Schizothorax prenanti

  • Mingrui Chen
  • Yinglong WuEmail author
  • Qiuping Yan
  • Jiaqi Zhao
  • Limei Feng
  • Mei He
  • Zhenzhen Lv
Original Article Aquaculture

Abstract

The aim of this study was to investigate the effects of dietary konjac oligosaccharide (KOS) supplements on the serum immune parameters, intestine microbiota, and intestine immunity of Schizothorax prenanti. Fish were fed a control diet (basal diet without KOS) or a diet with KOS supplements (0.4% KOS g kg−1 [low-KOS] and 1.6% KOS g kg−1 [high-KOS]. Fish on the high-KOS diet had significant increases in serum alternative hemolytic complement activity, complement 3 content, and nitric oxide content and a significant decrease in malondialdehyde content. Total superoxide dismutase activity was significantly in fish on KOS-supplemented diets, regardless of inclusion level. The lactic acid bacteria levels were significantly increased in fish on the high-KOS diet, while the Aeromonas populations were significantly decreased in fish on the KOS-supplemented diets, regardless of inclusion level. Lysozyme activity in both the mid and distal intestine was significantly enhanced by the high-KOS diet. Only the high-KOS diet significantly increased the acid phosphatase activity in the distal intestine. Regarding immune-related gene expressions, the high-KOS diet was observed to significantly induce the expression of Toll-like receptor 22 in the both anterior and distal intestine. Tumor necrosis factor-alpha mRNA level in the whole intestine was significantly up-regulated in fish on the KOS-supplemented diets, regardless of the inclusion level. The expression of Interleukin 1beta in both mid and distal intestine was significantly promoted by the KOS-supplemented diets, regardless of inclusion level.

Keywords

Konjac oligosaccharide Schizothorax prenanti Serum immune parameters Intestinal immunity Intestinal microbiota 

Notes

Author contributions

M-R. C. and Y-L. W. designed the experiment. M-R. C. with the help of M. H., Q-P. Y., J-Q. Z., L-M. F., and Z-Z. L. conducted the experiments. M-R. C. and Q-P. Y. analyzed the data. M-R. C. drafted the manuscript.

Compliance of ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Supplementary material

12562_2018_1252_MOESM1_ESM.doc (50 kb)
Supplementary material 1 (DOC 50 kb)

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Copyright information

© Japanese Society of Fisheries Science 2018

Authors and Affiliations

  1. 1.College of Food ScienceSichuan Agricultural UniversityYaanPeople’s Republic of China

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