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Fisheries Science

, Volume 83, Issue 4, pp 543–550 | Cite as

Sodium fluoride influences calcium metabolism resulting from the suppression of osteoclasts in the scales of nibbler fish Girella punctata

  • Masayuki Sato
  • Koji Yachiguchi
  • Keiichi Motohashi
  • Yukio Yaguchi
  • Yoshiaki Tabuchi
  • Yoichiro Kitani
  • Takahiro Ikari
  • Shouzo Ogiso
  • Toshio Sekiguchi
  • Tran Ngoc Hai
  • Do Thi Thanh Huong
  • Nguyen Viet Hoang
  • Makoto Urata
  • Hiroyuki Mishima
  • Atsuhiko Hattori
  • Nobuo SuzukiEmail author
Original Article Biology

Abstract

The influence of sodium fluoride (NaF) on calcium metabolism was examined in nibbler fish (marine teleosts). Two days after the administration of NaF (5 μg/g of body weight) (around 10−4 M in fish), we showed that plasma calcium levels significantly decreased in NaF-treated nibbler fish. In addition, we detected fluoride in the treated scales by use of a scanning electron microscope with an energy-dispersive X-ray microanalysis, indicating that NaF directly affects their scales. Therefore, the influence of NaF on osteoblasts and osteoclasts in the scales was examined. In the scales of NaF-injected nibbler fish, tartrate-resistant acid phosphatase (TRAP) (osteoclastic marker enzyme) decreased, although alkaline phosphatase (osteoblastic marker enzyme) was activated. To confirm the effect of NaF on osteoclasts, furthermore, the mRNA expressions of osteoclastic markers (matrix metalloproteinase-9 and TRAP) were decreased significantly 2 days after incubation. In barred knifejaws, plasma calcium levels decreased as they did in nibbler fish. Therefore, NaF functions in both osteoblasts and osteoclasts and then influences calcium metabolism in marine fish. In the marine environment, high levels of fluoride (1.2–1.5 mg F/l) (around 10−5–10−4 M) are present in seawater. It is probable that teleosts living in seawater efficiently use fluoride to regulate their blood calcium levels.

Keywords

Sodium fluoride Osteoblasts Osteoclasts Scales Calcium metabolism Element analysis Nibbler fish 

Notes

Acknowledgements

This study was supported in part by grants to N.S. (Grant-in-Aid for Scientific Research [C] No. 16K07871 by JSPS), to A.H. (Grant-in-Aid for Scientific Research [C] No. 24570068 by JSPS), to T.S. (Scientific Research [C] No. 15K07126 by JSPS), and to H.M. (Scientific Research [C] No. 15K11034 by JSPS). This work was performed under the cooperative research program of the Institute of Nature and Environmental Technology, Kanazawa University, Acceptance No. 17022, in 2017. We greatly appreciate Notojima and Uozu aquariums for providing us with barred knifejaw.

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Copyright information

© Japanese Society of Fisheries Science 2017

Authors and Affiliations

  • Masayuki Sato
    • 1
  • Koji Yachiguchi
    • 1
  • Keiichi Motohashi
    • 2
  • Yukio Yaguchi
    • 2
  • Yoshiaki Tabuchi
    • 3
  • Yoichiro Kitani
    • 1
  • Takahiro Ikari
    • 1
  • Shouzo Ogiso
    • 1
  • Toshio Sekiguchi
    • 1
  • Tran Ngoc Hai
    • 4
  • Do Thi Thanh Huong
    • 4
  • Nguyen Viet Hoang
    • 5
  • Makoto Urata
    • 1
    • 6
  • Hiroyuki Mishima
    • 7
  • Atsuhiko Hattori
    • 8
  • Nobuo Suzuki
    • 1
    Email author
  1. 1.Noto Marine Laboratory, Institute of Nature and Environmental TechnologyKanazawa UniversityNoto-choJapan
  2. 2.Electron Microscope CenterTokyo University of AgricultureTokyoJapan
  3. 3.Division of Molecular Genetics Research, Life Science Research CenterUniversity of ToyamaToyamaJapan
  4. 4.College of Aquaculture and FisheriesCan Tho UniversityCan ThoVietnam
  5. 5.Department of Science and TechnologyCenter of Application and InformationCamauVietnam
  6. 6.Institute of Noto SATOUMI Education ResearchNoto-choJapan
  7. 7.Department of Dental EngineeringTsurumi University School of Dental MedicineYokohamaJapan
  8. 8.Department of Biology, College of Liberal Arts and SciencesTokyo Medical and Dental UniversityIchikawaJapan

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