Fisheries Science

, Volume 83, Issue 1, pp 107–112 | Cite as

Genome size distribution in phylum Cnidaria

  • Kenta AdachiEmail author
  • Hiroshi Miyake
  • Takashi Kuramochi
  • Kanta Mizusawa
  • Sei-ichi Okumura
Original Article Aquaculture


Cnidarians are considered ancestral metazoans and, therefore, are important taxa for studying animal evolution. However, little is known about the group’s genome size (C value), which is an important parameter in whole-genome sequencing. To address this issue, we measured the C values of 27 cnidarian species from Japan, using flow cytometry, and found that they ranged from 0.26 to 3.56 pg. Excluding the results for Agalma elegans and Physalia physalis (order Siphonophorae), which had the highest C values among the species included in the present study, the C values for the cnidarians were 0.26–1.49 pg. In particular, we found that hydrozoans possessed relatively large and wide-ranging C values, indicating that evolution within the group involved considerable gains or losses of genomic content. Overall, the C values reported in the present study could be valuable for whole-genome sequencing, using next-generation sequencers, and for future research in cytogenetics.


Jellyfish Coral Sea anemone DNA content C value enigma 



The authors are grateful to Dr. Ryusuke Kado for species identification and advice and to Dr. Sho Toshino and Ms. Mari Takasawa for collecting Aurelia aurita s.l., Carybdea brevipedalia, and Turritopsis sp.


  1. 1.
    Swift H (1950) The constancy of desoxyribose nucleic acid in plant nuclei. P Natl Acad Sci USA 36:643–654CrossRefGoogle Scholar
  2. 2.
    Leroy S, Bouamer S, Morand S, Fargette M (2007) Genome size of plant-parasitic nematodes. Nematology 9:449–450CrossRefGoogle Scholar
  3. 3.
    Pedersen RA (1971) DNA content, ribosomal gene multiplicity, and cell size in fish. J Exp Zool 177:65–79CrossRefPubMedGoogle Scholar
  4. 4.
    Adachi K, Kuramochi T, Kimura K, Okumura S (2013) First extensive examination of genome size in phylum Brachiopoda (lamp shells) collected from Japan. J Shellfish Res 32:539–541CrossRefGoogle Scholar
  5. 5.
    Thomas CA (1971) The genetic organization of chromosomes. Annu Rev Genet 5:237–256CrossRefPubMedGoogle Scholar
  6. 6.
    Gregory TR (2005) The C value enigma in plants and animals: a review of parallels and an appeal for partnership. Ann Bot 95:133–146CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Vinogradov AE (1998) Genome size and GC-percent in vertebrates as determined by flow cytometry: the triangular relationship. Cytometry 31:100–109CrossRefPubMedGoogle Scholar
  8. 8.
    Gregory TR (2001) Coincidence, coevolution, or causation? DNA content, cell size, and the C value enigma. Biol Rev Camb Philos Soc 76:65–101CrossRefPubMedGoogle Scholar
  9. 9.
    Ohno S (1970) Gene dup1ication and the uniqueness of vertebrate genomes circa 1970–1999. Semin Cell Dev Biol 10:517–522CrossRefGoogle Scholar
  10. 10.
    Lundin LG (1993) Evolution of the vertebrate genome as reflected in paralogous chromosomal regions in man and the house mouse. Genomics 16:1–19CrossRefPubMedGoogle Scholar
  11. 11.
    Holland PWH, Garcia-Fernandez J, Williams NA, Sidow A (1994) Gene duplications and the origins of vertebrate development. Dev Suppl 1994:125–133Google Scholar
  12. 12.
    Daly M, Brugler MR, Cartwright P, Collins AG, Dawson MN, Fautin DG, France SC, McFadden CS, Opresko DM, Rodriguez E, Romano SL, Stake JL (2007) The phylum Cnidaria: a review of phylogenetic patterns and diversity 300 years after Linnaeus. Zootaxa 1668:127–182Google Scholar
  13. 13.
    Dumont HJ (1994) The distribution and ecology of the fresh- and brackish-water medusa of the world. Hydrobiologia 272:1–12CrossRefGoogle Scholar
  14. 14.
    Jakovčev-Todorović D, Đikanović V, Skorić S, Cakić P (2010) Freshwater jellyfish Craspedacusta sowerbyi Lankester, 1880 (Hydrozoa, Olindiidae): 50 years’ observations in Serbia. Arch Biol Sci 62:123–127CrossRefGoogle Scholar
  15. 15.
    Stepanjants SD (2013) Deep-water Hydrozoa (Cnidaria: Medusozoa) in the Sea of Japan, collected during the 51st Cruise of R/V Akademik M.A. Lavrentyev, with description Opercularella angelikae, sp. nov. Deep-Sea Res II 86:231–237CrossRefGoogle Scholar
  16. 16.
    Lucas CH, Graham WM, Widmer C (2012) Jellyfish life histories: role of polyps in forming and maintaining scyphomedusa populations. Adv Mar Biol 63:133–196CrossRefPubMedGoogle Scholar
  17. 17.
    Ronowicz M, Kukliński P, Mapstone GM (2015) Trends in the diversity, distribution and life history strategy of Arctic Hydrozoa (Cnidaria). PLoS One 10:e0120204CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Honda N, Matsushita Y, Watanabe T, Iizumi H (2005) The countermeasures for mitigating impacts of the giant jellyfish Nemopilema nomurai to fishing industries. Nippon Suisan Gakkaishi 71:975–976CrossRefGoogle Scholar
  19. 19.
    Yoshioka N, Kamizono M (2005) Recent increase of jellyfish populations and their nuisance to fisheries in the inland sea of Japan. Bull Fukuoka Fisheries Mar Technol Res Cent 15:89–94Google Scholar
  20. 20.
    Shinzato C, Shoguchi E, Kawashima T, Hamada M, Hisata K, Tanaka M, Fujie M, Fujiwara M, Koyanagi R, Ikuta T, Fujiyama A, Miller DJ, Satoh N (2011) Using the Acropora digitifera genome to understand coral responses to environmental change. Nature 476:320–323CrossRefPubMedGoogle Scholar
  21. 21.
    Goldberg RB, Crain WR, Ruderman JV, Moore GP, Barnett TR, Higgins RC, Gelfand RA, Galau GA, Britten RJ, Davidson EH (1975) DNA sequence organization in the genomes of five marine invertebrates. Chromosoma 51:225–251CrossRefPubMedGoogle Scholar
  22. 22.
    Mirsky AE, Ris H (1951) The desoxyribonucleic acid content of animal cells and its evolutionary significance. J Gen Physiol 34:451–462CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Zacharias H, Anokhin B, Khalturin K, Bosch TCG (2004) Genome sizes and chromosomes in the basal metazoan Hydra. Zoology 107:219–227CrossRefPubMedGoogle Scholar
  24. 24.
    Kubota S (2005) Distinction of two morphotypes of Turritopsis nutricula medusae (Cnidaria, Hydrozoa, Anthomedusae) in Japan, with reference to their different abilities to revert to the hydroid stage and their distinct geographical distributions. Biogeography 7:41–50Google Scholar
  25. 25.
    Adachi K, Okumura S (2012) Determination of genome size of Haliotis discus hannai and H. diversicolor aquatilis (Haliotidae) and phylogenetic examination of this family. Fish Sci 78:849–852CrossRefGoogle Scholar
  26. 26.
    Rees DJ, Belzile C, Glémet H, Dufresne F (2008) Large genomes among caridean shrimp. Genome 51:159–163CrossRefPubMedGoogle Scholar
  27. 27.
    Doležel J, Bartos J, Voglmayr H, Greilhuber J (2003) Nuclear DNA content and genome size of trout and human. Cytometry A 51:127–128CrossRefPubMedGoogle Scholar
  28. 28.
    Fukui Y (1996) Karyotype of the sea anemone Aiptasiomorpha sp. (Anthozoa, Actiniaria) from Japan. Biol Bull 190:6–7CrossRefGoogle Scholar
  29. 29.
    Heyward AJ (1985) Chromosomes of the coral Goniopora lobata (Anthozoa: Scieractinia). Heredity 55:269–271CrossRefGoogle Scholar
  30. 30.
    Kenyon JC (1997) Models of reticulate evolution in the coral genus Acropora based on chromosome numbers: parallels with plants. Evolution 51:756–767CrossRefGoogle Scholar
  31. 31.
    Diupotex-Chong ME, Ocaña-Luna A, Sánchez-Ramírez M (2009) Chromosome analysis of Aurelia aurita Linné, 1758 (Scyphozoa: Ulmaridae), southern Gulf of Mexico. Mar Biol Res 5:399–403CrossRefGoogle Scholar
  32. 32.
    Anokhin B, Hemmrich-Stanisak G, Bosch TCG (2010) Karyotyping and single-gene detection using fluorescence in situ hybridization on chromosomes of Hydra magnipapillata (Cnidaria: Hydrozoa). Comp Cytogenet 4:97–110CrossRefGoogle Scholar
  33. 33.
    Cartwright P, Nawrocki AM (2010) Character evolution in Hydrozoa (phylum Cnidaria). Integr Comp Biol 50:456–472CrossRefPubMedGoogle Scholar

Copyright information

© Japanese Society of Fisheries Science 2016

Authors and Affiliations

  • Kenta Adachi
    • 1
    Email author
  • Hiroshi Miyake
    • 1
  • Takashi Kuramochi
    • 2
  • Kanta Mizusawa
    • 1
  • Sei-ichi Okumura
    • 1
  1. 1.School of Marine BiosciencesKitasato UniversitySagamiharaJapan
  2. 2.Hayama Shiosai MuseumHayamaJapan

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