Cellulolytic activity in the hepatopancreas of Chionoecetes opilio and Chionoecetes japonicus: enzymatic adaptations to deep sea environment
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Chionoecetes opilio and Chionoecetes japonicus are closely related crab species that inhabit bathymetrically distinct habitats. C. opilio is caught mainly in waters shallower than 500 m, whereas C. japonicus largely resides at depths below 500 m. In this study, the activity of partially purified cellulase (β-1,4-glucanase) from the hepatopancreas of C. opilio (CoCel) and C. japonicus (CjCel) was investigated under high static pressure. SDS-PAGE-zymogram analysis revealed a major band in both species, with a calculated molecular mass of 41 kDa. In tests under various static pressures from 0.1 to 200 MPa, CoCel maintained 80 % of its activity up to 20 MPa, but activity decreased sharply to 20 % at 200 MPa in a pressure-dependent manner. In contrast, CjCel was less sensitive to high pressure, and maintained 65 % activity at 200 MPa. The activity of both CoCel and CjCel followed Michaelis–Menten kinetics at normal pressure levels; however, enzymatic activity of both CoCel and CjCel was suppressed in a non-competitive manner. These results suggest that CjCel, which can maintain normal activity under extremely high static pressure, have become highly adapted to the deep sea environment compared with CoCel.
KeywordsChionoecetes opilio Chionoecetes japonicus Cellulase Adaptation Deep sea Hydrostatic pressure
The authors would like to thank Professor Fumiyoshi Abe (Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University) for his useful advice. The authors are grateful to Hideaki Yamada from the Tottori Fisheries Experimental Station for kindly providing the experimental samples for preliminary study. This work was supported in part by the Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (JSPS KAKENHI; Grant Number 26520308).
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