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Fisheries Science

, Volume 82, Issue 3, pp 459–471 | Cite as

Spatial and temporal variability of unicellular diazotrophic cyanobacteria in the eastern Seto Inland Sea

  • Ryoya Hashimoto
  • Hiroyasu Watai
  • Kazutaka Miyahara
  • Yoshihiko Sako
  • Takashi YoshidaEmail author
Original Article Biology

Abstract

The Seto Inland Sea is one of the largest semi-enclosed seas of Japan, where inflows of both nutrient-rich freshwater and nutrient-poor open ocean water have a profound influence on oceanographic conditions in the surface layers. To explore the diversity and distribution of unicellular diazotrophic cyanobacteria in this highly complex coastal area, we evaluated the seasonal dynamics of the cyanobacteria in the Seto Inland Sea over a period of 2 years. We obtained sequences belonging to UCYN-A and UCYN-C from the UCYN 16S rRNA gene clone library. The UCYN-C sequences were divided into three major types, referred to as UCYN-C ribotypes 1, 2, and 3; these groups clustered with Cyanothece ATCC51142, Cyanothece SKTU126, and the endosymbiont of Rhopalodia gibba, respectively. Results of qPCR showed that UCYN-A was detected during the early summer season at sampling points with temperatures ranging from 14 to 28 °C and with relatively nitrogen-depleted conditions. The highest abundance of UCYN-C was observed during the winter season, reaching 8.3 × 105 nifH gene copies l−1. Our results revealed a widespread presence of unicellular diazotrophic cyanobacteria in the Seto Inland Sea, suggesting their contribution to the biogeochemical cycle.

Keywords

Seto Inland Sea Unicellular diazotrophic cyanobacteria UCYN-A UCYN-C 

Notes

Acknowledgments

We are grateful to the captain and crew of the research vessel Shin-Hyogo (Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries). We thank Mr. Kazuhiro Harada (Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries) for his cooperation with the measurement of water samples. This work was supported by JSPS KAKENHI grants no. 23658160 and 14J02468.

Supplementary material

12562_2016_983_MOESM1_ESM.docx (152 kb)
Supplementary material 1 (DOCX 151 kb)
12562_2016_983_MOESM2_ESM.docx (66 kb)
Supplementary material 2 (DOCX 65 kb)

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Copyright information

© Japanese Society of Fisheries Science 2016

Authors and Affiliations

  • Ryoya Hashimoto
    • 1
  • Hiroyasu Watai
    • 1
  • Kazutaka Miyahara
    • 2
  • Yoshihiko Sako
    • 1
  • Takashi Yoshida
    • 1
    Email author
  1. 1.Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Hyogo Prefectural Technology Center for Agriculture, Forestry and FisheriesFisheries Technology InstituteAkashiJapan

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