Fisheries Science

, Volume 79, Issue 2, pp 321–326 | Cite as

Characterization of vitamin B12 compounds from the brackish-water bivalve Corbicula japonica

  • Yukio Ishihara
  • Kazumi Ueta
  • Tomohiro Bito
  • Shigeo Takenaka
  • Yukinori Yabuta
  • Fumio WatanabeEmail author
Original Article Chemistry and Biochemistry


In Lake Togo (Tottori prefecture, Japan), the vitamin B12 content of the brackish-water bivalve Corbicula japonica remained high (17.3–22.5 μg/100 g wet weight of the edible portion) throughout the year, except during August and September. We evaluated whether the bivalve contained true vitamin B12 or other corrinoid compounds that are inactive in humans by purifying the compounds and identifying them using LC/ESI–MS/MS chromatograms. The results indicated that “true” vitamin B12 predominated in the bivalve, although small amounts of inactive corrinoid compounds (pseudovitamin B12 and three unidentified compounds) were also found.


Brackish-water bivalve Corbicula japonica Corrinoid compound Vitamin B12 


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Copyright information

© The Japanese Society of Fisheries Science 2013

Authors and Affiliations

  • Yukio Ishihara
    • 1
    • 2
  • Kazumi Ueta
    • 1
    • 3
  • Tomohiro Bito
    • 1
  • Shigeo Takenaka
    • 4
  • Yukinori Yabuta
    • 1
  • Fumio Watanabe
    • 1
    Email author
  1. 1.The United Graduate School of Agricultural SciencesTottori UniversityTottoriJapan
  2. 2.Tottori Prefecture Fisheries Experimental StationSakai-MinatoJapan
  3. 3.Department of Science for Human HealthShikoku University Junior CollegeTokushimaJapan
  4. 4.Graduate School of Life and Environmental SciencesOsaka Prefecture UniversityOsakaJapan

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