Fisheries Science

, Volume 78, Issue 3, pp 603–611

Microsatellite multiplex panels for population genetic analysis of red sea bream Pagrus major

  • Enrique Blanco Gonzalez
  • Masaki Aritaki
  • Nobuhiko Taniguchi
Original Article Aquaculture

DOI: 10.1007/s12562-012-0469-5

Cite this article as:
Blanco Gonzalez, E., Aritaki, M. & Taniguchi, N. Fish Sci (2012) 78: 603. doi:10.1007/s12562-012-0469-5

Abstract

In this study, multiplex PCR panels were developed to amplify 20 microsatellite markers for red sea bream, Pagrus major, with the aim of reducing labor and material costs associated with genetic analysis of this species. The usefulness of these panels was validated in 200 fish collected at five sampling locations. The occurrence of null alleles was suggested at five markers, which were dropped from further analysis. The remaining 15 microsatellite loci showed high levels of heterozygosity (HE range 0.34–0.97, HO range 0.32–1) and a wide range in the number of alleles per locus (A; range 5–46). Increasing the number of microsatellite loci from three to ten and 15 improved the performance of the panels for population differentiation (FST) and estimation of Nei’s (DS) genetic distance. The results of this study confirm the usefulness of genotyping a large number of microsatellite DNA markers to expand our knowledge of red sea bream population genetics.

Keywords

Multiplex Microsatellite Red sea bream Pagrus major Stock enhancement Genetic diversity Population structure 

Copyright information

© The Japanese Society of Fisheries Science 2012

Authors and Affiliations

  • Enrique Blanco Gonzalez
    • 1
  • Masaki Aritaki
    • 2
  • Nobuhiko Taniguchi
    • 1
  1. 1.The Research Institute of Marine Bioresources of Fukuyama UniversityOnomichiJapan
  2. 2.Coastal Fisheries and Aquaculture Division, Seikai National Fisheries Research InstituteFisheries Research AgencyNagasakiJapan

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