Fisheries Science

, Volume 78, Issue 1, pp 197–206 | Cite as

Species identification method for marine products of Seriola and related species

  • Jun Iguchi
  • Yasuharu Takashima
  • Atsushi Namikoshi
  • Michiaki Yamashita
Original Article Food Science and Technology

Abstract

The complete nucleotide sequences of mitochondrial DNA (mtDNA) from four Seriola spp. (S. quinqueradiata, S. lalandi, S. dumerili, and S.rivoliana) were determined with the aim of developing a species identification analysis method for discriminating between commercially important Seriola spp. and other related species. In addition, the nucleotide sequences of the mitochondrial cytochrome b gene (Cytb) from five related but less expensive species in terms of market value (Seriolella brama, S. caerulea, S. punctata, Hyperoglyphejaponica, and Rachycentroncanadum), which are often used as substitutes for Seriola spp., were determined. Restriction enzyme sites were examined by comparing the nucleotide sequences, and species-specific primers were designed for PCR-based restriction fragment length polymorphism (RFLP) analysis. Based on the results of the PCR amplification studies, the four Seriola spp. and the five related species tested could be categorized into three groups according to their PCR product pattern: a 373-bp product from the four Seriola spp., a 513-bp product from three Seriolella spp. and H. japonica, and a 204-bp product from R. canadum. In addition, RFLP analysis of the PCR products was able to differentiate these fish species.

Keywords

Hyperoglyphejaponica PCR–RFLP Rachycentroncanadum Seriola spp. Seriolella spp. Species identification 

References

  1. 1.
    Balanov AA (2008) On the species composition of fish of the genus Seriola (Carandigae) in the northwestern part of the sea of Japan. J Ichthyol 48:415–421CrossRefGoogle Scholar
  2. 2.
    Gushiken S (1983) Revision of the carangid fishes of Japan. Galaxea 2:135–264Google Scholar
  3. 3.
    The Ministry of Agriculture, Forestry, Fisheries of Japan (MAFF) (2010) Statistics of Agriculture, Forestry and Fisheries in 2010 (in Japanese). MAFF, TokyoGoogle Scholar
  4. 4.
    Norman JR (1937) Coast fishes Part II. The Patagonia region. Discov Rep 16:115–116Google Scholar
  5. 5.
    Hadrich RL (1967) The Stromateoid fishes: systematics and a classification. Bull Mus Comp Zool 135:69–72Google Scholar
  6. 6.
    Cousseau MB, Forciniti L, Ubaldi G (1993) Species of the genus Seriolella (Centrolophidae) in Southwest Atlantic Waters. Jpn J Ichtyol 40(2):183–187Google Scholar
  7. 7.
    Sezaki K, Itoi S, Watabe S (2005) A simple method to distinguish two commercially valuable eel species in Japan Anguilla japonica and A. anguilla using polymerase chain reaction strategy with a species-specific primer. Fish Sci 71:414–421CrossRefGoogle Scholar
  8. 8.
    Takashima Y, Morita T, Yamashita M (2006) Complete mitochondrial DNA sequence of Atlantic horse mackerel Trachurus trachurus and molecular identification of two commercially important species T. trachurus and T. japonicus using PCR-RFLP. Fish Sci 72:1054–1065CrossRefGoogle Scholar
  9. 9.
    Russell VJ, Hold GL, Pryde SE, Rehbein H, Quinteiro J, Rey-Mendez M, Sotelo CG, Pérez-Martín RI, Santos AT, Rosa C (2000) Use of restriction fragment length polymorphism to distinguish between salmon species. J Agric Food Chem 48:2184–2188PubMedCrossRefGoogle Scholar
  10. 10.
    Nakabo T (2000) Fishes of Japan with pictorial keys to the species, 2nd edn (in Japanese). Tokai University Press, Tokyo, pp 792–793, 960Google Scholar
  11. 11.
    Kocher TD, Thomas WK, Meyer A, Edwards SV, Pääbo S, Villablanca FX, Wilson AC (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc Natl Acad Sci USA 86:6196–6200PubMedCrossRefGoogle Scholar
  12. 12.
    Miya M, Nishida M (2000) Use of mitogenomic information in teleostean molecular phylogenetics: a tree-based exploration under the maximum-parsimony optimality criterion. Mol Phylogenet Evol 17:437–455PubMedCrossRefGoogle Scholar
  13. 13.
    Gutell RR, Gray MW, Schnare MN (1993) A compilation of large subunit (23S and 23S-like) ribosome RNA structures. Nucleic Acids Res 21:3055–3074PubMedCrossRefGoogle Scholar
  14. 14.
    Bibb MJ, Van Etten RA, Wright CT, Walberg MW, Clayton DA (1981) Sequence and gene organization of mouse mitochondrial DNA. Cell 26:167–180PubMedCrossRefGoogle Scholar
  15. 15.
    Anderson S, de Bruijn MHL, Coulson AR, Eperon IC, Sanger F, Young IG (1982) Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome. J Mol Biol 156:683–717PubMedCrossRefGoogle Scholar
  16. 16.
    Inoue JG, Miya M, Tsukamoto K, Nishida M (2000) Complete mitochondrial DNA sequence of the Japanese sardine Sardinops melanostictus. Fish Sci 66:924–932CrossRefGoogle Scholar
  17. 17.
    Inoue JG, Miya M, Aoyama J, Ishikawa S, Tsukamoto K, Nishida M (2001) Complete mitochondrial DNA sequence of the Japanese eel Anguilla japonica. Fish Sci 67:118–125CrossRefGoogle Scholar
  18. 18.
    Inoue JG, Miya M, Tsukamoto K, Nishida M (2001) Complete mitochondrial DNA sequence of the Japanese anchovy Engraulis japonicus. Fish Sci 67:828–835CrossRefGoogle Scholar
  19. 19.
    Coulson MW, Marshall HD, Pepin P, Carr SM (2006) Mitochondrial genomics of gadine fishes: implications for taxonomy and biogeographic origins from whole-genome data sets. Genome 49:1115–1130PubMedCrossRefGoogle Scholar
  20. 20.
    Quinteiro J, Vidal R, Izquierdo M, Sotelo CG, Chapela MJ, Pérez-Martín RI, Rehbein H, Hold GL, Russell VJ, Pryde SE, Rosa C, Santos AT, Rey-Méndez M (2001) Identification of Hake Species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences. J Agric Food Chem 49:5108–5114PubMedCrossRefGoogle Scholar

Copyright information

© The Japanese Society of Fisheries Science 2011

Authors and Affiliations

  • Jun Iguchi
    • 1
  • Yasuharu Takashima
    • 1
  • Atsushi Namikoshi
    • 2
  • Michiaki Yamashita
    • 3
  1. 1.Food and Agricultural Materials Inspection CenterChuo-ku, SaitamaJapan
  2. 2.Food and Agricultural Materials Inspection Center, Kobe Regional CenterChuo-ku, KobeJapan
  3. 3.National Research Institute of Fisheries ScienceFisheries Research AgencyKanazawa-ku, YokohamaJapan

Personalised recommendations