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Fisheries Science

, Volume 77, Issue 4, pp 657–664 | Cite as

Isolation and characterization of Noctiluca-killing bacteria from a shrimp aquaculture pond in Thailand

  • Teeyaporn KeawtaweeEmail author
  • Kimio Fukami
  • Putth Songsangjinda
  • Pensri Muangyao
Original Article Environment

Abstract

To control harmful algal blooms (HABs), in particular dinoflagellate Noctiluca scintillans, which causes damage to shrimp production, algicidal bacteria were isolated from shrimp pond water and screened for their killing effect against N. scintillans under laboratory condition. Among 260 bacterial isolates, 10 showed killing effects on N. scintillans. Out of 5 strains showing relatively stronger killing activity, 4 strains belonged to the Gammaproteobacteria group. Among these, strain BS2 (identified as Marinobacteria salsuginis) showed the strongest Noctiluca-killing activity. In the present study, we also investigated bacterial effects against growth of other phytoplankton species. Strain BS2 killed only N. scintillans but not any other phytoplankton (Heterosigma akashiwo NIES-6, Chatonella antique NIES-1, Chaetoceros ceratosporum, Prorocentrum lima, and Dunaliella sp.). Results of the present study lead us to expect prevention of damage by HABs, in particular dinoflagellate N. scintillans, in shrimp farming and mitigation for shrimp production in ponds using Noctiluca-killing bacteria.

Keywords

Algicidal bacteria Noctiluca scintillans HABs Shrimp aquaculture 

Notes

Acknowledgments

This study was supported by the Ministry of Education, Culture, Sports, Science, and Technology (Monbukagakusho), Japanese Government, JSPS, and the Fund of the President of Kochi University. We are thankful to the staff of the Coastal Aquaculture Research Institute, Songkhla and Dumrong Farm for their valuable help with fieldwork and for collecting the samples.

References

  1. 1.
    Alonso RR, Osuna PF (2003) Nutrients, phytoplankton and harmful algal blooms in shrimp ponds: a review with special reference to the situation in the Gulf of California. Aquaculture 219:317–336CrossRefGoogle Scholar
  2. 2.
    Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402PubMedCrossRefGoogle Scholar
  3. 3.
    Chen YQ, Gu XG (1993) An ecological study of red tides in the East China Sea. In: Smayda TJ, Schimizu L (eds) Toxic phytoplankton blooms in the sea. Elsevier, Amsterdam, pp 217–221Google Scholar
  4. 4.
    Clinton EH, Elif D, Cary SC, Kathryn CJ, Kirchman DL, David AH (2005) A bacterium that inhibits the growth of Pfiesteria piscicida and other dinoflagellates. Harmful Algae 4:221–234CrossRefGoogle Scholar
  5. 5.
    Doucette GJ (1995) Interactions between bacteria and harmful algae: a review. Nat Toxins 3:65–74PubMedCrossRefGoogle Scholar
  6. 6.
    Doucette GJ, Kodama M, Franca S, Gallacher S (1998) Bacterial interactions with harmful algal bloom species: bloom ecology, toxigenesis, and cytology. In: Anderson DM, Cembella AD, Hallegraeff GM (eds) Physiological ecology of harmful algal blooms. Springer, Berlin, pp 619–647Google Scholar
  7. 7.
    Doucette GJ, McGovern ER, Babinchak JA (1999) Algicidal bacteria active against Gymnodinium breve (Dinophyceae). I. Bacterial isolation and characterization of killing activity. J Phycol 35:1447–1454CrossRefGoogle Scholar
  8. 8.
    Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  9. 9.
    Fukami K, Nishijima T, Hata Y (1992) Availability of deep seawater and effects of a bacteria isolated from deep seawater on the mass culture of food microalga Chaetoceros ceratosporum. Nippon Suisan Gakkaishi 58:931–936Google Scholar
  10. 10.
    Fukami K, Nishijima T, Murata H, Doi S, Hata Y (1991) Distribution of bacteria influential on the development and the decay of Gymnodinium nagasakiense red tide and their effect on algal growth. Nippon Suisan Gakkaishi 57:2321–2326Google Scholar
  11. 11.
    Fukami K, Yuzawa A, Nishijima T, Hata Y (1992) Isolation and properties of a bacterium inhibiting the growth of Gymnodinium nagasakiense. Nippon Suisan Gakkaishi 58:1073–1077Google Scholar
  12. 12.
    Fukami K, Yuzawa A, Sakaguchi K, Nishijima T (1995) Prevention of noxious red tide by algicidal bacteria. In: Proceedings of International conference on Ecological System Enhancement Technology for Aquatic Environments, Japan International Science and Technology Federation, Tokyo, pp 89–94Google Scholar
  13. 13.
    Imai I, Ishida Y, Hata Y (1993) Killing of marine phytoplankton by a gliding bacterium Cytophaga sp., isolated from the coastal Sea of Japan. Mar Biol 116:527–532CrossRefGoogle Scholar
  14. 14.
    Imai I, Ishida Y, Sawayama K, Hata Y (1991) Isolation of marine gliding bacterium that kills Chattonella antique (Raphidophyceae). Nippon Suisan Gakkaishi 57:1409Google Scholar
  15. 15.
    Imai I, Kim MC, Nagasaki K, Itakura S, Ishida Y (1998) Relationships between dynamics of red tide-causing raphidophycean flagellates and algicidal micro-organisms in the coastal sea of Japan. Phycol Res 46:139–146CrossRefGoogle Scholar
  16. 16.
    Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120PubMedCrossRefGoogle Scholar
  17. 17.
    Mayali X, Azam F (2004) Algicidal bacteria in the sea and their impact on algal blooms. J Eukaryot Microbiol 51:139–144PubMedCrossRefGoogle Scholar
  18. 18.
    Riquelme CE, Fukami K, Ishida Y (1988) Effects of bacteria on the growth of a marine diatom, Asterionella glacialis. Bull Jpn Soc Microb Ecol 3:29–34Google Scholar
  19. 19.
    Salomon PS, Imai I (2006) Pathogens of harmful algae. In: Granéli E, Turner JT (eds). Ecology of harmful algae. Ecological studies. Springer, Berlin, pp 271–282Google Scholar
  20. 20.
    Songsangjinda P, Yamamoto T, Fukami K, Keawtawee T (2006) Importance of controlling community structure of living organisms in intensive shrimp culture ponds. Coast Mar Sci 30:91–99Google Scholar
  21. 21.
    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680PubMedCrossRefGoogle Scholar
  22. 22.
    Yoshinaga I, Kawai T, Ishida Y (1997) Analysis of algicidal ranges of the bacteria killing the marine dinoflagellate Gymnodinium mikimotoi isolated from Tanabe Bay, Wakayama Pref. Jpn Fish Sci 63:94–98Google Scholar

Copyright information

© The Japanese Society of Fisheries Science 2011

Authors and Affiliations

  • Teeyaporn Keawtawee
    • 1
    Email author
  • Kimio Fukami
    • 1
  • Putth Songsangjinda
    • 2
  • Pensri Muangyao
    • 3
  1. 1.Graduate School of Kuroshio ScienceKochi UniversityKochiJapan
  2. 2.Trang Coastal Aquaculture StationTrangThailand
  3. 3.Coastal Aquaculture Research InstituteSongkhlaThailand

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