Fisheries Science

, Volume 76, Issue 6, pp 981–990 | Cite as

Myxosporean and microsporidian infections in cultured Pacific bluefin tuna Thunnus orientalis in Japan

  • Jinyong Zhang
  • Fei Meng
  • Hiroshi Yokoyama
  • Jiro Miyahara
  • Ikuo Takami
  • Kazuo Ogawa
Original Article Aquaculture

Abstract

During the parasitological survey of cultured juvenile Pacific bluefin tuna (PBT) Thunnus orientalis in 2007 and 2008, two myxosporeans and one microsporidian were found. Morphological and molecular analysis showed that the heart-infecting and brain-infecting myxosporeans are identified as Kudoa shiomitsui and K. yasunagai, respectively. This is a new host record for both species. High prevalence of infection (77–100%) with K. shiomitsui was observed in October to December (1–2 months post-transfer to sea cages), whereas only a few fish were infected with K. yasunagai. A microsporidian observed as white “cysts” in the trunk muscle of PBT had a resemblance to Microsporidium seriolae, the causative parasite of beko disease in yellowtail. Phylogenetic analysis showed that the microsporidian from PBT is closely related to but distinct from several other muscle-infecting species such as M. seriolae, Microsporidium sp. RSB, and Microsporidium sp. SH. Additionally, the spore dimension (2.7 × 1.5 μm on average) was remarkably smaller than the others. These results suggest that the present microsporidian is an undescribed species and designated Microsporidium sp. PBT. Prevalence and intensity of infection with Microsporidium sp. PBT reached a maximum of 100% and 20 cysts/fish, respectively. Although pathogenic effects of the two Kudoa species on fish health remain unknown, the microsporidian could be of concern to PBT aquaculture due to unsightly cysts in the musculature, reducing the market value of the fish.

Keywords

Kudoa shiomitsui Kudoa yasunagai Microsporidium Pacific bluefin tuna Thunnus orientalis Myxosporean Microsporidian 

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Copyright information

© The Japanese Society of Fisheries Science 2010

Authors and Affiliations

  • Jinyong Zhang
    • 1
  • Fei Meng
    • 2
  • Hiroshi Yokoyama
    • 2
  • Jiro Miyahara
    • 3
  • Ikuo Takami
    • 3
  • Kazuo Ogawa
    • 2
  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhanPeople’s Republic of China
  2. 2.Department of Aquatic Bioscience, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  3. 3.Nagasaki Prefectural Institute of FisheriesNagasakiJapan

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