Fisheries Science

, Volume 76, Issue 3, pp 481–487 | Cite as

Enhancement of Penaeus monodon shrimp postlarvae growth and survival without water exchange using marine Bacillus pumilus and periphytic microalgae

  • Sanjoy Banerjee
  • Helena Khatoon
  • Mohamed Shariff
  • Fatimah Md. Yusoff
Original Article Aquaculture
First Online:
Received:
Accepted:

Abstract

We have investigated the possibility of using a consortium of marine bacterium and periphytic microalgae to improve the water quality and increase the growth and survival of the shrimp Penaeus monodon in a hatchery system. Three treatments were evaluated for their effect on P. monodon postlarvae (PL) when the culture water was not changed: Bacillus pumilus alone (B); periphytic microalgae alone (M); B. pumilus + periphytic microalgae (BM). P. monodon PL raised in a tank of unchanged water without bacterium and periphytic microalgae served as the control. The water in tanks of the M and BM treatments had significantly low levels of total ammonia-nitrogen (TAN) (0.03 and 0.01 mg l−1, respectively) and nitrite-nitrogen (NO2-N) (0.03, 0.01 mg l−1, respectively) than that in the B (TAN 0.80, NO2-N 0.68 mg l−1) and control (TAN 1.11, NO2-N 1.12 mg l−1) tanks. Moreover, PL cultured in tanks M and BM had significantly higher survival and specific growth rates and a significantly higher resistance to the reverse salinity stress test than those in the B and control tanks. Compared to the control PL, the PL cultured in the BM tanks had significantly higher levels of protein, lipid, polyunsaturated fatty acids, ecosapentaenoic acid, and docosahexaenoic acid. The culture water in tanks BM also contained significantly less Vibrio than the control water. Our results illustrate the beneficial effects of a B. pumilus and periphytic microalgae consortium on improving the water quality and the growth and survival of shrimp PL grown in a hatchery system.

Keywords

Bacillus pumilus Consortium Penaeus monodon Periphytic microalgae Postlarvae 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This study was supported by the National Biotechnology Directorate (NBD) Malaysia project no. 01-02-04-002 BTK/ER/004 and Research University Grant Scheme (RUGS), Universiti Putra Malaysia, project no. 05/01/07/0181RU.

References

  1. 1.
    Shishehchian F, Yusoff FM, Omar H, Kamarudin MS (1999) Nitrogenous excretion of Penaeus monodon postlarvae fed with different diets. Mar Pollut Bull 39:224–227CrossRefGoogle Scholar
  2. 2.
    Ziemann DA, Walsh WA, Saphore EG, Fulton-Bennet K (1992) A survey of water quality characteristics of effluent from Hawaiian aquaculture facilities. J World Aquac Soc 23:180–191CrossRefGoogle Scholar
  3. 3.
    Lalloo R, Ramchuran SO, Ramduth DM, Gorgens J (2007) Isolation and selection of Bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish. J Appl Microbiol 103:1471–1479Google Scholar
  4. 4.
    Rengpipat S, Phianphak W, Piyatiratitivorakul S, Menasveta P (1998) Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167:301–313CrossRefGoogle Scholar
  5. 5.
    Aujero EJ, Tech E, Javellana S (1985) Nutritional value of marine yeast fed to larvae of Penaeus monodon in combination with algae. In: Taki Y et al (eds) Proc First Int Conf Culture Penaeid Prawns/Shrimp. South-East Asian Fisheries Development Center, IloiloGoogle Scholar
  6. 6.
    Austin B, Stuckey LF, Robertson PAW, Effendi I, Griffith DRW (1995) A probiotic strain of Vibrio alginolyticus effective in reducing diseases caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordalii. J Fish Dis 18:93–96CrossRefGoogle Scholar
  7. 7.
    Coutteau P, Geurden I, Camara MR, Bergot P, Sorgeloos P (1997) Review on the dietary effects of phospholipids in fish and crustacean larviculture. Aquaculture 155:149–164CrossRefGoogle Scholar
  8. 8.
    Yúfera M, Lubián LM (1990) Effects of microalgal diets on growth and development of invertebrates in marine aquaculture. In: Akatsuka I (ed) Introduction to applied phycology. SPB Academic Publ, The Hague, pp 209–227Google Scholar
  9. 9.
    Brown MR, Jeffrey SW, Volkman JK, Dunstan GA (1997) Nutritional properties of microalgae for mariculture. Aquaculture 151:315–331CrossRefGoogle Scholar
  10. 10.
    Duerr EO, Molnar A, Sato V (1998) Cultured microalgae as aquaculture feed. J Mar Biol 6:65–70Google Scholar
  11. 11.
    Dersjant-Li Y (2002) The use of soy protein in aquafeeds. In: Cruz-Suarez LE et al (eds) Avances en nutricion acuıcola VI. Memorias del VI Simposium Internacional de Nutricion Acuıcola. Cancu′n, Quintana Roo, pp 541–558Google Scholar
  12. 12.
    Gross A, Boyd CE, Wood CE (2000) Nitrogen transformations and balance in channel catfish. Aquacult Eng 24:1–14CrossRefGoogle Scholar
  13. 13.
    Asaduzzaman M, Wahab MA, Verdegem MCJ, Huque S, Salam MA, Azim ME (2008) C/N ratio control and substrate addition for periphyton development jointly enhance freshwater prawn Macrobrachium rosenbergii production in ponds. Aquaculture 280:117–123CrossRefGoogle Scholar
  14. 14.
    Khatoon H, Yusoff FM, Banerjee S, Shariff M, Mohamed S (2007) Use of periphytic cyanobacterium and mixed diatoms coated substrate for improving water quality, survival and growth of Penaeus monodon Fabricius postlarvae. Aquaculture 271:196–205CrossRefGoogle Scholar
  15. 15.
    Khatoon H, Banerjee S, Yusoff FM, Shariff M (2009) Evaluation of indigenous marine periphytic Amphora, Navicula and Cymbella grown on substrate as feed supplement in Penaeus monodon postlarval hatchery system. Aquacult Nutr 15:186–193CrossRefGoogle Scholar
  16. 16.
    Devaraja TN (2002) Investigation of indigenous Bacillus isolates with bioremediation properties for improving water quality and shrimp health in Malaysian aquaculture. PhD thesis. Universiti Putra Malaysia, MalaysiaGoogle Scholar
  17. 17.
    Tompkins J, Deville MM, Day JG, Turner MF (1995) Culture collection of algae and protozoa. Catalogue of strains, AmblesideGoogle Scholar
  18. 18.
    Parsons TR, Maita Y, Laili CM (1984) A manual of chemical and biological methods for seawater analysis. Pergamon Press, New YorkGoogle Scholar
  19. 19.
    Khatoon H (2006) Use of selected periphyton species to improve the water quality and shrimp postlarval production. PhD thesis. Universiti Putra Malaysia, MalaysiaGoogle Scholar
  20. 20.
    Meyer E, Walther A (1988) Methods for the estimation of protein, lipid, carbohydrates and chitin levels in freshwater invertebrates. Arch Hydrobiol 113:161–177Google Scholar
  21. 21.
    Divakaran S, Ostrowski AC (1989) Fatty acid analysis of fish eggs without solvent extraction. Aquaculture 80:371–375CrossRefGoogle Scholar
  22. 22.
    Gomez KA, Gomez AA (1983) Statistical procedure for agricultural research. Wiley, New YorkGoogle Scholar
  23. 23.
    Zar JH (1984) Biostatistical analysis, 2nd edn. Prentice-Hall, New YorkGoogle Scholar
  24. 24.
    Williams PJ, Le B (1981) Incorporation of microheterotrophic processes into the classical paradigm of the planktonic food web. Kiel Meeresforsch Son-derh 4:1–28Google Scholar
  25. 25.
    Mevel G, Prieur D (2000) Heterotrophic nitrification by a thermophilic Bacillus species as influenced by different culture conditions. Can J Microbiol 46:465–473CrossRefPubMedGoogle Scholar
  26. 26.
    Kim JK, Park KJ, Cho KS, Nam S-W, Park T-J, Bajpai R (2005) Aerobic nitrification–denitrification by heterotrophic Bacillus strains. Bioresour Technol 96:1897–1906CrossRefPubMedGoogle Scholar
  27. 27.
    Bender J, Phillips P (2004) Microbial mats for multiple applications in aquaculture and bioremediation. Bioresour Technol 94:229–238CrossRefPubMedGoogle Scholar
  28. 28.
    Jawahar Abraham T, Sasmal D, Ghosh S (2004) Development and efficacy of bioremediators for ammonia removal in shrimp aquaculture. In: Abidi SAH et al (eds) Proc Natl Seminar New Frontiers Mar Biosci Res. Allied, India, pp 69–75Google Scholar
  29. 29.
    Gomez-Gil B, Roque A, Turnbull JF (2000) The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture 191:259–270CrossRefGoogle Scholar
  30. 30.
    Verschuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671CrossRefPubMedGoogle Scholar
  31. 31.
    Moriarty DJW (1998) Control of luminous Vibrio species in penaeid aquaculture ponds. Aquaculture 164:351–358CrossRefGoogle Scholar
  32. 32.
    Banerjee S, Devaraja TN, Shariff M, Yusoff FM (2007) Comparison of four antibiotics with native marine Bacillus spp. in controlling pathogenic bacteria from shrimp and Artemia. J Fish Dis 30:383–389CrossRefPubMedGoogle Scholar
  33. 33.
    Siddhanta AK, Mody KH, Ramavat BK, Chauhan VD, Garg HS, Goel AK, Jinandra Doss M, Srivastava MN, Patnaik GK, Kamboj VP (1997) Bioactivity of marine organisms. Part VIII. Screening of some marine flora of Western coast of India. Indian J Exp Biol 35:638–643PubMedGoogle Scholar
  34. 34.
    Marques A, Thanh TH, Sorgeloos P, Bossier P (2006) Use of microalgae and bacteria to enhance protection of gnotobiotic Artemia against different pathogens. Aquaculture 258:116–126CrossRefGoogle Scholar
  35. 35.
    Schumacher G, Blume T, Sekoulov I (2003) Bacteria reduction and nutrient removal in small wastewater treatment plants by an algal biofilm. Water Sci Technol 47:195–202PubMedGoogle Scholar
  36. 36.
    Brown MR, Dunstan GA, Norwood SJ, Miller KA (1996) Effects of harvest stage and light on the biochemical composition of the diatom Thalassiosira pseudonana. J Phycol 32:64–73CrossRefGoogle Scholar
  37. 37.
    Thompson FL, Abreu PC, Wasielesky W (2002) Importance of biofilm for water quality and nourishment in intensive shrimp culture. Aquaculture 203:263–278CrossRefGoogle Scholar
  38. 38.
    Ballester ELC, Wasielesky W Jr, Cavalli RO, Abreu PC, Santos MHS (2004) Biofilm improve nursery culture of pink shrimp in Brazil. G A A 7:8Google Scholar
  39. 39.
    Rengpipat S, Tunyanum A, Piyatiratitivorakul S, Menasaveta P (2003) Enhanced growth and resistance to Vibrio challenge in pond-reared black tiger shrimp Penaeus monodon fed a Bacillus probiotic. Dis Aquat Org 55:169–173CrossRefPubMedGoogle Scholar
  40. 40.
    Lovett DL, Felder DL (1990) Ontogenic change in digestive enzyme activity of larval and postlarval white shrimp Penaeus setiferus (Crustacea, Decapoda, Penaeidae). Biol Bull 178:144–159CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Fisheries Science 2010

Authors and Affiliations

  • Sanjoy Banerjee
    • 1
  • Helena Khatoon
    • 1
  • Mohamed Shariff
    • 1
  • Fatimah Md. Yusoff
    • 1
  1. 1.Institute of BioscienceUniversiti Putra Malaysia, UPMSerdangMalaysia

Personalised recommendations