Fisheries Science

, Volume 75, Issue 3, pp 657–663 | Cite as

Henneguya pseudorhinogobii n. sp. (Myxozoa: Myxosporea) parasitizing the gills of the freshwater goby Rhinogobius sp. OR from the Nagara River and redescription of Henneguya rhinogobii

  • Tetsushi Kageyama
  • Tetsuya Yanagida
  • Kenichi Ohara
  • Hiroshi Yokoyama
Original Article Biology


Two types of infection with myxosporean parasites, which were different in cyst size, were found from the gill of Rhinogobius sp. OR collected from the Nagara River, Gifu Prefecture, Japan. A myxosporean forming large-type cysts in the gill filament was morphologically identified as Henneguya rhinogobii. Another myxosporean forming small-type cysts was found to be parasitic in the gill lamella. Average spore sizes of the latter myxosporean were 15.8 (range 14.2–17.8) μm in length, 5.3 (4.7–5.8) μm in width, 6.5 (5.9–7.6) μm in polar capsule length and 34.9 (25.3–42.9) μm in caudal appendage length, which were almost identical to those of H. rhinogobii. However, the two were distinguishable by the location in the gill (intrafilamental or intralamellar) and by the difference in the sequence of SSU rDNA (about 96% similarity). We propose the myxosporean forming intralamellar small-type cysts as Henneguya pseudorhinogobii n. sp. and redescribe H. rhinogobii by morphological and molecular studies.


Henneguya pseudorhinogobii Henneguyarhinogobii Myxosporean Myxozoa Nagara River New species Rhinogobius sp. OR 


  1. 1.
    Yokoyama H, Kageyama T, Ohara K, Yanagida T (2007) Myxobolus nagaraensis n. sp. (Myxozoa: Myxosporea) causes abdominal distension of freshwater goby Rhinogobius sp. OR type from the Nagara River. Fish Sci 73:633–639CrossRefGoogle Scholar
  2. 2.
    Akihito, Sakamoto K, Ikeda Y, Iwata A (2002) Gobiidae. In: Nakabo T (eds) Fishes of Japan with Pictorial Keys to the Species. Tokai University Press, Tokyo, pp 1139–1310, 1606–1628Google Scholar
  3. 3.
    Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–154CrossRefGoogle Scholar
  4. 4.
    Hillis DM, Dixon MT (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Quart Rev Biol 66:411–453PubMedCrossRefGoogle Scholar
  5. 5.
    Freeman M, Yokoyama H, Ogawa K (2008) Description and phylogeny of Ceratomyxa anko sp. n. and Zschokkella lophii sp. n. from the Japanese anglerfish. J Fish Dis (in press)Google Scholar
  6. 6.
    Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882PubMedCrossRefGoogle Scholar
  7. 7.
    Swofford DL (1999) PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4.0. Sinauer associates, SunderlandGoogle Scholar
  8. 8.
    Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120PubMedCrossRefGoogle Scholar
  9. 9.
    Molnár K (2002) Site preference of fish myxosporeans in the gill. Dis Aquat Org 48:197–207PubMedCrossRefGoogle Scholar
  10. 10.
    Eiras JC (2002) Synopsis of the species of the genus Henneguya Thelohan, 1892 (Myxozoa: Myxosporea: Myxobolidae). Syst Parasitol 52:43–54PubMedCrossRefGoogle Scholar
  11. 11.
    Yokoyama H, Itoh N, Tanaka S (2005) Henneguya pagri n. sp. (Myxozoa: Myxosporea) causing cardiac henneguyosis in red sea bream, Pagrus major. J Fish Dis 28:479–487PubMedCrossRefGoogle Scholar
  12. 12.
    Casal G, Matos E, Azevedo C (1997) Some ultrastructural aspects of Henneguya striolata sp. nov. (Myxozoa, Myxosporea) a parasite of the Amazonian fish Serrasalmus striolatus. Parasitol Res 83:93–95PubMedCrossRefGoogle Scholar
  13. 13.
    Reed CC, Basson L, Van As LL (2003) Myxozoans infecting the sharptooth catfish, Clarias gariepinus in the Okavango River and Delta, Botswana, including descriptions of two new species, Henneguya samochimensis sp. n. and Myxobolus gariepinus sp. n. Folia Parasitol 50:183–189PubMedGoogle Scholar
  14. 14.
    Adriano EA, Arana S, Cordeiro NS (2005) Histopathology and ultrastructure of Henneguya caudalongula sp. n. infecting Prochilodus lineatus (Pisces: Prochilodontidae) cultivated in the state of Sao Paulo, Brazil. Mem Inst Oswaldo Cruz 100:177–181PubMedCrossRefGoogle Scholar
  15. 15.
    Awakura T, Tonosaki H (1978) Studies on Henneguya spp. (Protozoa: Myxosporidia) parasitic in gobiid fishes. Sci Rep Hokkaido Fish Hatch 31:101–115Google Scholar
  16. 16.
    Chen CL (1973) Classification of pathogens. In: Illustration of Fish Pathogens in Hubei Province. Science Press, Beijing, pp 91–92 (in Chinese)Google Scholar
  17. 17.
    Chen CL, Ma CL (1998) Myxozoa Myxosporea. In: Fauna Sinica. Science Press, Beijing, p 538 (in Chinese)Google Scholar
  18. 18.
    Molnár K, Marton SZ, Eszterbauer E, Székely CS (2006) Comparative morphological and molecular studies on Myxobolus spp. infecting chub from the River Danube, Hungary, and description of M. muellericus sp. n. Dis Aquat Org 73:49–61PubMedCrossRefGoogle Scholar
  19. 19.
    Eszterbauer E (2002) Molecular biology can differentiate morphologically indistinguishable myxosporean species: Myxobolus elegans and M. hungaricus. Acta Vet Hung 50:59–62PubMedCrossRefGoogle Scholar
  20. 20.
    Eszterbauer E, Marton S, Racz OZ, Letenyei M, Molnár K (2006) Morphological and genetic differences among actinosporean stages of fish-parasitic myxosporeans (Myxozoa): difficulties of species identification. Syst Parasitol 65:97–114PubMedCrossRefGoogle Scholar
  21. 21.
    Haaparanta A, Valtonen ET, Hoffmann RW (1994) Pathogenicity and seasonal occurrence of Henneguya creplini (Protozoa, Myxosporea) on gills of perch Perca fluviatilis in central Finland. Dis Aquat Org 20:15–22CrossRefGoogle Scholar
  22. 22.
    Nakamura M (1969) Cyprinid fishes of Japan: studies on the life history of Cyprinid fishes of Japan. Research Institute for Natural Resources, Tokyo (in Japanese)Google Scholar

Copyright information

© The Japanese Society of Fisheries Science 2009

Authors and Affiliations

  • Tetsushi Kageyama
    • 1
  • Tetsuya Yanagida
    • 2
  • Kenichi Ohara
    • 1
  • Hiroshi Yokoyama
    • 3
  1. 1.Gifu Prefectural Research Institute for Freshwater Fish and Aquatic EnvironmentsKakamigaharaJapan
  2. 2.Department of ParasitologyAsahikawa Medical CollegeHokkaidoJapan
  3. 3.Department of Aquatic Biosciences, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyoJapan

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