Fisheries Science

, Volume 75, Issue 2, pp 379–385 | Cite as

Comparison between surface-reading and cross-section methods using sagittal otolith for age determination of the marbled sole Pseudopleuronectes yokohamae

  • Jeong-Hoon LeeEmail author
  • Keita Kodama
  • Gen Kume
  • Masaaki Oyama
  • Satoshi Katayama
  • Yuji Takao
  • Toshihiro Horiguchi
Original Article Biology


To find an appropriate method for age determination in the marbled sole Pseudopleuronectes yokohamae in Tokyo Bay, Japan, sagittal otoliths of 1,343 individuals were observed by surface-reading and cross-section methods and the results were compared. Opaque zones occurred once a year and were regarded as annuli in both methods. The surface-reading method sometimes provided a lower count of the number of annuli than the cross-section method, and the frequency of this discrepancy was highest in older fish (males above 5 years, females above 4 years). The oldest female fish was estimated to be age 10 years by the cross-section method but 8 years by the surface-reading method. The cross-section method could provide a more accurate estimate of age and is therefore likely to be indispensable to estimations of longevity. In contrast, the surface-reading method is superior in terms of cost and time efficiency but is likely to underestimate the ages of older fish. However, growth equations based on age estimated by the surface-reading method were sufficiently accurate if males ≥5 years and females ≥4 years were combined as specific, single age groups of 5+ and 4+, respectively.


Age determination Cross-section method Growth Otolith Pseudopleuronectes yokohamae Surface-reading method Tokyo Bay 



We are grateful to Mr. Tatsuya Isshiki of the Kanagawa Prefectural Fisheries Technology Center for providing some of the samples. We would also like to thank the commercial fishermen of Yokosuka, Shiba, and Koitogawa for their help in sampling.


  1. 1.
    Sakamoto K (1984) Pleuronectidae righteye flounders. In: Masuda H, Amoaoka K, Araga C, Ueno T, Yoshino T (eds) The fishes of the Japanese archipelago. Tokai University Press, TokyoGoogle Scholar
  2. 2.
    Kodama K, Aoki I, Shimizu M, Taniuchi T (2002) Long-term changes in the assemblage of demersal fishes and invertebrates in relation to environmental variations in Tokyo Bay, Japan. Fish Manag Ecol 9:303–313CrossRefGoogle Scholar
  3. 3.
    Kume G, Horiguchi T, Goto A, Shiraishi H, Shibata Y, Morita M, Shimizu M (2006) Seasonal distribution, age, growth, and reproductive biology of marbled sole Pleuronectes yokohamae in Tokyo Bay, Japan. Fish Sci 72:289–298CrossRefGoogle Scholar
  4. 4.
    Beamish RJ, McFarlane GA (1983) The forgotten requirement for age validation in fisheries biology. Trans Am Fish Soc 112:735–743CrossRefGoogle Scholar
  5. 5.
    Campana SE (2001) Accuracy, precision and quality control in age determination, including a review of the use and abuse of age validation methods. J Fish Biol 59:197–242CrossRefGoogle Scholar
  6. 6.
    Solomon G, Sano M, Shimizu M, Nose Y (1987) Age and growth of the pleuronectid flounder Limanda yokohamae in Tokyo Bay, Japan. Nippon Suisan Gakkaishi 53:711–716Google Scholar
  7. 7.
    Park JS (1988) A study on the pleuronectid flounder Limanda yokohamae population in Tokyo Bay. Ph.D. dissertation, University of Tokyo, Tokyo (in Japanese)Google Scholar
  8. 8.
    Shafieipour MM, Takahashi T, Ishino K, Takatsu T, Nakatani T (1999) Age and growth of marbled sole Pleuronectes yokohamae in Kikonai Bay, Hokkaido, Japan. Iran J Fish Sci 1:5–21Google Scholar
  9. 9.
    Tanda M, Gorie S, Nakamura Y, Okamoto S (2008) Age and growth of marbled sole Pleuronectes yokohamae in Harima Nada and Osaka Bay, the Seto Inland Sea, Japan. Nippon Suisan Gakkaishi 74:1–7 (in Japanese with English abstract)CrossRefGoogle Scholar
  10. 10.
    Beamish RJ (1979) Differences in the age of Pacific hake (Merluccius productus) using whole otoliths and sections of otoliths. J Fish Res Board Can 36:141–151Google Scholar
  11. 11.
    Hayashi A, Dotsu K, Ohta M (1995) Reliability of surface reading methods to count otolith opaque zones on aging in marbled rockfish Sebastiscus marmoratus compared with the cutaway reading method. Nippon Suisan Gakkaishi 61:1–5 (in Japanese with English abstract)Google Scholar
  12. 12.
    Haas RE, Recksiek CW (1995) Age verification of winter flounder in Narragansett Bay. Trans Am Fish Soc 124:103–111CrossRefGoogle Scholar
  13. 13.
    Peltonen H, Raitaniemi J, Parmanne R, Eklund J, Nyberg K, Halling F (2002) Age determination of Baltic herring from whole otoliths and from neutral red stained otolith cross sections. ICES J Mar Sci 59:323–332CrossRefGoogle Scholar
  14. 14.
    Dwyer KS, Walsh SJ, Campana SE (2003) Age determination, validation and growth of Grand Bank yellowtail flounder (Limanda ferruginea). ICES J Mar Sci 60:1123–1138CrossRefGoogle Scholar
  15. 15.
    Sekigawa T, Takahashi T, Takatsu T, Nishiuchi S, Sasaki M, Shiokawa F (2003) Age and growth of Sebastes vulpes in the coastal waters of western Hokkaido, Japan. Fish Sci 69:575–580CrossRefGoogle Scholar
  16. 16.
    Atsuchi S, Masuda Y, Akamo H, Iori K (2004) Age and growth of Japanese flounder Paralichthys olivaceus from the coastal waters around Kagoshima Prefecture, Japan, using transverse sections of otoliths. Nippon Suisan Gakkaishi 70:714–721 (in Japanese with English abstract)CrossRefGoogle Scholar
  17. 17.
    Doiuchi R, Kokubo T, Ogawa M (2007) Age and growth of threeline grunt Parapristipoma trilineatum along the south-western coast of Kii Peninsula, Japan. Fish Sci 73:777–783CrossRefGoogle Scholar
  18. 18.
    Yabuki K (1989) Age determination of yanagimushigarei Tanakius kitaharai (Pleuronectidae) from otoliths in the sea of Japan off Kyoto Prefecture. Nippon Suisan Gakkaishi 55:1331–1338Google Scholar
  19. 19.
    Polat N, Bostanci D, Yilmaz S (2001) Comparable age determination in different bony structures of Pleuronectes flesus luscus Pallas, 1811 inhabiting the Black Sea. Turk J Zool 25:441–446Google Scholar
  20. 20.
    Forsberg JE (2001) Aging manual for Pacific halibut: procedures and methods used at the international pacific halibut commission (IPHC). IPHC, SeattleGoogle Scholar
  21. 21.
    Beamish RJ, Fournier DA (1981) A method for comparing the precision of a set of age determinations. Can J Fish Aquat Sci 38:982–983CrossRefGoogle Scholar
  22. 22.
    Powers JE (1983) Some statistical characteristic of ageing data and their ramifications in population analysis of ocean pelagic fish. NOAA Tech Rep NMFS 8:19–24Google Scholar
  23. 23.
    Tokai T (1997) Maximum likelihood parameter estimates of a mesh selectivity logistic model through Solver on MS-Excel. Bull Jpn Soc Fish Oceanogr 61:288–298 (in Japanese with English abstract)Google Scholar
  24. 24.
    Gorie S (2001) Estimation of the parameters in von Bertalanffy’s growth formula by MS-Excel. Suisanzoshoku 49:519–527 (in Japanese with English abstract)Google Scholar
  25. 25.
    Akamine T (2004) Statistical test and model selection of fish growth formula. Bull Jpn Soc Fish Oceanogr 68:44–51 (in Japanese with English abstract)Google Scholar

Copyright information

© The Japanese Society of Fisheries Science 2009

Authors and Affiliations

  • Jeong-Hoon Lee
    • 1
    • 2
    Email author
  • Keita Kodama
    • 1
  • Gen Kume
    • 3
  • Masaaki Oyama
    • 1
  • Satoshi Katayama
    • 4
  • Yuji Takao
    • 5
  • Toshihiro Horiguchi
    • 1
  1. 1.Research Center for Environmental RiskNational Institute for Environmental StudiesTsukubaJapan
  2. 2.Graduate School of Science and TechnologyNagasaki UniversityNagasakiJapan
  3. 3.Landcare ResearchLincolnNew Zealand
  4. 4.Coastal Fisheries and Aquaculture DivisionNational Research Institute of Fisheries ScienceNagaiJapan
  5. 5.Faculty of Environmental StudiesNagasaki UniversityNagasakiJapan

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