Effect of juglone on the survival time of two Brachionus species (Rotifera): species-specific tolerance against oxidative stress
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To investigate the effect of oxidative stress on the survival of two Brachionus species of rotifer, we examined the effective dose of juglone, which generates reactive oxygen species, and compared survival times between the two species. First, we observed that juglone affected survival in the rotifers in a dose-dependent manner between 0.02 to 20 μM, and that treatment at above 2 μM showed acute toxicity causing the animals to die within a few hours. Next, we found the difference in survival time between the two species exposed to 20 μM juglone: B. rotundiformis was significantly more tolerant against the substance than the allied species B. plicatilis. The findings suggest that exploring the basis of species-specific abilities to persist under oxidative stress will be of great interest to uncover the underlying mechanisms governing the stress resistance of the rotifer, as well as contributing to their stable mass production for aquaculture.
KeywordsBrachionus Juglone Oxidative stress Rotifer
The authors wish to thank two anonymous reviewers for their critical reading of our manuscript, and Drs. Atsushi Hagiwara and Koushiro Suga of Nagasaki University for providing the rotifer JS strain. This study was partly supported by a Grant-in-Aid for Young Scientist (A) no. 18688010 from the Ministry of Education, Culture, Sports, and Science and Technology of Japan, and a Research Grant for Young Researchers from the Kitasato University to T.Y.
- 10.Przybysz AJ, Choe KP, Strange K, Roberts LJ (2007) Ageing inhibits the adaptive response of Caenorhabditis elegans to the reactive oxygen species-generating compound juglone. Free Rad Biol Med 43:158Google Scholar
- 11.Cypser JR, Johnson TE (2002) Multiple stressors in Caenorhabditis elegans induce stress hormesis and extended longevity. J Gerontol 57:109–114Google Scholar
- 12.Kampkötter A, Volkmann TE, de Castro SH, Leiers B, Klotz L-O, Johnson TE, Link CD, Henkle-Dührsen K (2003) Functional analysis of the glutathione S-transferase 3 from Onchocerca volvulus (Ov-GST-3): a parasite GST confers increased resistance to oxidative stress in Caenorhabditis elegans. J Mol Biol 325:25–37PubMedCrossRefGoogle Scholar
- 14.Yoshinaga T, Minegishi Y, Rumengan IFM, Kaneko G, Furukawa S, Yanagawa Y, Tsukamoto K, Watabe S (2004) Molecular phylogeny of the rotifers with two Indonesian Brachionus lineages. Coast Mar Sci 29:45–56Google Scholar
- 19.Gallardo WG, Tomita Y, Hagiwara A, Soyano K, Snell TW (1997) Effect of dimethysulfoxide (DMSO), sodium hydroxide (NaOH), acetone, and ethanol on the population growth, mictic female production, and body size of the rotifer Brachionus plicatilis Muller. Bull Fac Fish Nagasaki Univ 78:15–22Google Scholar