The First Detection of Human Bocavirus Species 2 and 3 in Raw Sewage and Mussels in South Africa
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Human bocavirus (HBoV) has a global distribution and is associated with respiratory and enteric infections, particularly in the paediatric population. In this study, raw sewage and mussel samples were analysed for the presence of HBoV using nested PCR with primers targeting the VP1/VP2 junction. Amplification and sequencing of the 382 bp region followed by phylogenetic analysis indicated the presence of HBoV 2 in mussel samples and HBoV 3 in sewage samples. This is the first report describing the presence of enteric-associated HBoV in environmental samples from South Africa and in mussel samples from the African continent. The results signify the need for further studies examining the potential risk of foodborne transmission of HBoV and highlight the importance of continued screening to determine the prevalence and epidemiology of HBoV in South Africa.
KeywordsHuman bocavirus Parvoviridae Wastewater Shellfish Enteric
This work was supported by Medical Research Council (MRC, South Africa) and Research Council (RC, Rhodes University) grants. OO was supported by the Department of Public Health, Ministry of Health and wellness, Botswana. NU was supported by postgraduate fellowships from the National Research Foundation of South Africa and the German Academic Exchange Service (DAAD). The content of this publication is solely the responsibility of the authors and does not necessarily represent official views of the funders.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Ethics approval for this work was not required as the study only involved invertebrates.
- Bergallo, M., Dapra, V., Rassu, M., Calvi, C., Montanari, P., & Galliano, I. (2019). Human bocavirus in children with acute gastroenteritis in Piedmont, Italy. Minerva Pediatrica. https://doi.org/10.23736/S0026-4946.18.05365-3
- Blinkova, O., Rosario, K., Li, L., Kapoor, A., Slikas, B., Bernardin, F., et al. (2009). Frequent detection of highly diverse variants of Cardiovirus, Cosavirus, Bocavirus, and Circovirus in sewage samples collected in the United States. Journal of Clinical Microbiology,47(11), 3507–3513. https://doi.org/10.1128/JCM.01062-09.CrossRefPubMedPubMedCentralGoogle Scholar
- Chhabra, P., Payne, D. C., Szilagyi, P. G., Edwards, K. M., Staat, M. A., Shirley, S. H., et al. (2013). Etiology of viral gastroenteritis in children < 5 years of age in the United States, 2008–2009. Journal of Infectious Diseases,208(5), 790–800. https://doi.org/10.1093/infdis/jit254.CrossRefPubMedGoogle Scholar
- Kapoor, A., Simmonds, P., Slikas, E., Li, L., Bodhidatta, L., Sethabutr, O., et al. (2010). Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. The Journal of Infectious Diseases,201(11), 1633–1643. https://doi.org/10.1086/652416.CrossRefPubMedPubMedCentralGoogle Scholar
- La Rosa, G., Sanseverino, I., Della Libera, S., Iaconelli, M., Ferrero, V. E. V., Barra Caracciolo, A., et al. (2017). The impact of anthropogenic pressure on the virological quality of water from the Tiber River Italy. Letters in Applied Microbiology,65(4), 298–305. https://doi.org/10.1111/lam.12774.CrossRefPubMedGoogle Scholar
- Lekana-Douki, S. E., Behillil, S., Enouf, V., Leroy, E. M., & Berthet, N. (2018). Detection of human bocavirus-1 in both nasal and stool specimens from children under 5 years old with influenza-like illnesses or diarrhea in Gabon. BMC Research Notes,11(495), 1–7. https://doi.org/10.1186/s13104-018-3605-1.CrossRefGoogle Scholar
- Onosi, O. (2018). Recovery and molecular identification of Aichi Virus 1, enteric human bocaviruses and enteric human adenoviruses in untreated sewage and mussel samples collected in the Eastern Cape Province of South Africa. Rhodes University.Google Scholar
- Purpari, G., Macaluso, G., Di, S., Gucciardi, F., Mira, F., Di, P., et al. (2019). Molecular characterization of human enteric viruses in food, water samples, and surface swabs in Sicily. International Journal of Infectious Diseases,80, 66–72. https://doi.org/10.1016/j.ijid.2018.12.011.CrossRefPubMedGoogle Scholar
- Rikhotso, M. C., Kabue, J. P., Ledwaba, S. E., Traoré, A. N., & Potgieter, N. (2018). Prevalence of human bocavirus in Africa and other developing countries between 2005 and 2016: A potential emerging viral pathogen for diarrhea. Journal of Tropical Medicine. https://doi.org/10.1155/2018/7875482.CrossRefGoogle Scholar
- Subramoney, K., Hellferscee, O., Pretorius, M., Tempia, S., McMorrow, M., von Gottberg, A., et al. (2018). Human bocavirus, coronavirus, and polyomavirus detected among patients hospitalised with severe acute respiratory illness in South Africa, 2012 to 2013. Health Science Reports,1(e59), 1–7. https://doi.org/10.1002/hsr2.59.CrossRefGoogle Scholar
- Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research,22(22), 4673–4680. https://doi.org/10.1093/nar/22.22.4673.CrossRefPubMedPubMedCentralGoogle Scholar