Food and Environmental Virology

, Volume 11, Issue 1, pp 96–100 | Cite as

The First Molecular Detection of Aichi Virus 1 in Raw Sewage and Mussels Collected in South Africa

  • Oikwathaile Onosi
  • Nicole S. UpfoldEmail author
  • Michael D. Jukes
  • Garry A. Luke
  • Caroline Knox
Brief Communication


Aichi virus 1 (AiV-1) has a worldwide distribution and is associated with gastroenteritis in humans. In this study, raw sewage and mussel samples were analyzed for the presence of AiV-1 using reverse transcription-PCR (RT-PCR). Amplification and sequencing of the 3CD and VP1 genomic regions followed by phylogenetic analysis using selected genome sequences revealed the presence of AiV-1, genotype B. The results highlight the importance of further screening to evaluate the prevalence and epidemiology of this clinically important virus in South Africa.


Aichi virus 1 Kobuvirus Mussels Waste water Picornaviridae 



This work was supported by Medical Research Council (MRC, South Africa) and Research Council (RC, Rhodes University) grants. OO was supported by the Department of Public Health, Ministry of Health and wellness, Botswana. NU was supported by postgraduate fellowships from the National Research Foundation of South Africa and the German Academic Exchange Service (DAAD). The content of this publication is solely the responsibility of the authors and does not necessarily represent official views of the funders.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics Approval

Ethics approval for this work was not required as the study only involved invertebrates.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biochemistry and MicrobiologyRhodes UniversityGrahamstownSouth Africa
  2. 2.Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences BuildingUniversity of St AndrewsSt AndrewsUK

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