Isolation and Characterization of Bacillus cereus Bacteriophages from Foods and Soil
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The aim of this study was to isolate and characterize Bacillus cereus bacteriophages of various origins. Twenty-seven bacteriophages against B. cereus were isolated from various Korean traditional fermented foods and soils. Plaque size, transmission electron microscopy, virulence profile, and in vitro lytic activity of bacteriophage isolates were examined. Transmission electron microscopy confirmed B. cereus bacteriophages belonging to the family Siphoviridae. Among B. cereus bacteriophages with broad host range, 18 isolates (66.7%) did not harbor any B. cereus virulence factors. Among them, bacteriophage strain CAU150036, CAU150038, CAU150058, CAU150064, CAU150065, and CAU150066 effectively inhibited B. cereus in vitro within 1 h. Therefore, they are considered potential candidates for controlling the contamination of B. cereus in food or other applications.
KeywordsBacteriophages Bacillus cereus Isolation Characterization Virulence factor Inhibition
This study was supported by the National Institute of Animal Science Rural Development Administration (Project No. 20130058), Republic of Korea and the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through (High Value-added Food Technology Development Program), funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (313023-3).
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Conflict of interest
Authors declare that there is no conflict of interest.
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