Food and Environmental Virology

, Volume 9, Issue 3, pp 350–353 | Cite as

Quantification and Genotyping of Aichi Virus 1 in Water Samples in the Kathmandu Valley, Nepal

  • Eiji HaramotoEmail author
  • Masaaki Kitajima
Brief Communication


Aichi virus 1 genomes were detected by quantitative PCR in groundwater from shallow dug (10/22) and tube wells (1/15), river water (14/14), and sewage (1/1), with the maximum concentration of 4.0 × 109 copies/l. Nucleotide sequencing analysis demonstrated the prevalence of genotype B in the virus positive samples.


Aichi virus 1 Groundwater Kathmandu Valley Picornaviridae 



This study was partially supported by the Grant-in-Aid for the Global COE Program “Evolution of Research and Education on Integrated River Basin Management in Asian Region” from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Interdisciplinary Center for River Basin Environment, Graduate Faculty of Interdisciplinary ResearchUniversity of YamanashiKofuJapan
  2. 2.Division of Environmental Engineering, Faculty of EngineeringHokkaido UniversitySapporoJapan

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