Food and Environmental Virology

, Volume 7, Issue 2, pp 159–163 | Cite as

Prevalence of Hepatitis E Virus in Populations of Wild Animals in Comparison with Animals Bred in Game Enclosures

  • Monika Kubankova
  • Petr Kralik
  • Jiri Lamka
  • Vladimir Zakovcik
  • Marek Dolanský
  • Petra Vasickova
Original Paper


Hepatitis E virus (HEV) is now accepted as a zoonotic virus, and domestic pigs, wild boars and deer are recognised as natural reservoirs of the pathogen. In this study, 762 animals (wild boars, fallow deer, red deer, sika deer, roe deer and mouflons) originating from the wild and from game enclosures were tested for the presence of HEV RNA by qRT-PCR. HEV RNA was detected in wild boars (96/450), red deer (2/169), roe deer (1/30) and mouflons (5/39). The sequence relationship between HEV isolates from wild boars and domestic pigs or humans indicate a circulation of HEV in the Czech Republic.


Captive animals Free-living animals Real-time RT-PCR Sequence analysis Zoonosis Epizootology 



The authors would like to thank Neysan Donnelly (Max-Planck-Institute of Biochemistry, Germany) for grammatical corrections of the manuscript. The results of the Project LO1218 were obtained with financial support from the MEYS of the CR under the NPU I program and supported by Grants NT13884-4/2012 and QJ1210113.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Monika Kubankova
    • 1
  • Petr Kralik
    • 1
  • Jiri Lamka
    • 2
  • Vladimir Zakovcik
    • 3
  • Marek Dolanský
    • 3
  • Petra Vasickova
    • 1
  1. 1.Veterinary Research InstituteBrnoCzech Republic
  2. 2.Faculty of Pharmacy in Hradec KraloveCharles University in PragueHradec KraloveCzech Republic
  3. 3.Central Military Veterinary Institute at HlucinHlucinCzech Republic

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