First Serological Study of Hepatitis E Virus Infection in Backyard Pigs from Serbia
- 90 Downloads
Hepatitis E virus (HEV) genotype 3 infections in pigs and humans have been lately reported in Europe. In the present study, the prevalence of anti-HEV IgG antibodies in swine was investigated, for the first time, in Serbia by means of an ELISA based on a recombinant open reading frame 2 protein of HEV genotype 3. A total of 315 serum samples from 3 to 4 months-old healthy backyard pigs, collected in 63 herds from 28 towns and villages of 4 different districts of the Vojvodina province, in the northern part of Serbia, were tested. A 34.6% (109/315) of the sera tested were positive. The prevalence of anti-HEV antibodies varied widely between municipalities (range 16.7–75.0%) and herds (range 0–100%). These data indicate that HEV infection is widespread in Serbian backyard pigs.
KeywordsHEV ELISA Backyard pigs Seroprevalence Serbia
The study was supported in part by grant (CSD2006-0007) from the Spanish Ministerio de Ciencia e Innovación (MICINN) to JCS and grant (TR 20115) from Serbian Ministry of Science and Technological Development to SL. NJO has been supported by a scholarship from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Two COST-929 short term missions supported training visits of DL and NJO.
- Bouwknegt, M., Engel, B., Herremans, M. M. P. T., Widdowson, M. A., Worm, H. C., Koopmans, M. P. G., et al. (2007). Bayesian estimation of hepatitis E virus seroprevalence for populations with different exposure levels to swine in the Netherlands. Epidemiology and Infection, 136(4), 567–576.PubMedGoogle Scholar
- Geng, Y., Wang, C., Zhao, C., Yu, X., Harrison, T.J., Tian, K., et al. (2009). Serological prevalence of hepatitis E virus in domestic animals and diversity of genotype 4 hepatitis E virus in China. Vector Borne Zoonotic Diseases. doi: 10.1089/vbz.2009.0168.
- Jiménez de Oya, N., Galindo, I., Escribano-Romero, E., Blázquez, A. B., Alonso-Padilla, J., Halaihel, N., et al. (2009a). Expression and immunoreactivities of hepatitis E virus (HEV) genotype 3 open reading frame-2 (ORF-2) recombinant proteins expressed in insect cells. Food and Environmental Virology, 1(2), 77–84.CrossRefGoogle Scholar
- Jiménez de Oya, N., Galindo, I., Gironés, O., Duizer, E., Escribano, J. M., & Saiz, J. C. (2009b). Serological immunoassay for detection of hepatitis E virus on the basis of genotype 3 open reading frame 2 recombinant proteins produced in Trichoplusia ni larvae. Journal of Clinical Microbiology, 47(10), 3276–3282.CrossRefPubMedGoogle Scholar
- Meng, X. J., Purcell, R. H., Halbur, P. G., Lehman, J. R., Webb, D. M., Tsareva, T. S., et al. (1997). A novel virus in swine is closely related to the human hepatitis E virus. Proceedings of the National Academy of Sciences USA, 94(18), 9860–9865.Google Scholar
- Meng, X. J., Wiseman, B., Elvinger, F., Guenette, D. K., Toth, T. E., Engle, R. E., et al. (2002). Prevalence of antibodies to hepatitis E virus in veterinarians working with swine and in normal blood donors in the United States and other countries. Journal of Clinical Microbiology, 40(1), 117–122.CrossRefPubMedGoogle Scholar
- Petrovic, T., Prodanov, J., Lazic, S. (2008). First preliminary results on the presence of Hepatitis E virus in swine population in Serbia. Symposium on Current Developments in Food and Environmental Virology, Pisa, Italy, (pp. 52–53).Google Scholar
- Pintó, R., & Saiz, J. C. (2007). Human viruses in water. In A. Bosch (Ed.), Perspectives in medical virology (Vol. 17, pp. 39–67). Amsterdam, The Netherlands: Elsevier.Google Scholar
- Yazaki, Y., Mizuo, H., Takahasi, M., Nishizawa, T., Sasaki, N., Gotanda, Y., et al. (2003). Sporadic acute hepatitis E in Hokkaido, Japan, may be food-borne, as suggested by the presence of hepatitis E virus in pig liver as food. Journal of General Virology, 84(9), 2351–2357.CrossRefPubMedGoogle Scholar
- Yoo, D., Wilson, P., Pei, Y., Hayes, M. A., Deckert, A., Dewey, C. E., et al. (2001). Prevalence of Hepatitis E Virus antibodies in Canadian swine herds and identification of a novel variant of swine Hepatitis E virus. Clinical and Diagnostic Laboratory Immunology, 8(6), 1213–1219.PubMedGoogle Scholar